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| 假丝酵母E-2产生物表面活性剂摇瓶发酵条件的优化 | |
| 引用本文： | 程永刚,周玲,王世明,于言平,肖中元,李玉林,秦丽.假丝酵母E-2产生物表面活性剂摇瓶发酵条件的优化[J].生物加工过程,2011,9(4):15-21. |
| 作者姓名： | 程永刚 周玲 王世明 于言平 肖中元 李玉林 秦丽 |
| 作者单位： | 1. 南京理工大学生物工程系,南京,210094;太极集团西南药业股份有限公司,重庆,400038 2. 太极集团西南药业股份有限公司,重庆,400038 3. 南京理工大学生物工程系,南京,210094 |
| 基金项目： | 国家自然科学基金资助项目 |
| 摘 要： | 从扬子石化的废水淤泥中筛选到1株能发酵液体石蜡产脂肽类生物表面活性剂的假丝酵母Candida E-2.通过单因子实验和正交试验,得到了最佳发酵培养基组成(g/L):牛肉膏3.0,蔗糖2.0,酵母膏0.25,KH2PO4 12.5,MgSO4 0.3,NaCl 1.5,CaCl,0.05,尿素0.5 5;液体石蜡10％(体积分数).最佳培养条件:初始pH7.0,接种量0.12g/L,装液量为200mL三角瓶30mL,培养时间为5 d.最终产量提高了2.7倍,达1.582g/L. |
| 关 键 词： | 生物表面活性剂 假丝酵母 发酵 优化 |
Optimization of shaking flask fermentation condition of biosurfactant by Candia E-2 |
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| CHENG Yonggang,ZHOU Ling,WANG Shiming,YU Yanping,XIAO Zhongyuan,LI Yulin,QIN Li.Optimization of shaking flask fermentation condition of biosurfactant by Candia E-2[J].Chinese Journal of Bioprocess Engineering,2011,9(4):15-21. | |
| Authors: | CHENG Yonggang ZHOU Ling WANG Shiming YU Yanping XIAO Zhongyuan LI Yulin QIN Li |
| Institution: | CHENG Yonggang1,2,ZHOU Ling2,WANG Shiming1,YU Yanping2,XIAO Zhongyuan2,LI Yulin2,QIN Li2(1.Department of Bioengineering,Nanjing University of Science and Technology,Nanjing 210094,China,2.Southwest Pharmaceutical Co.,Ltd.,Taiji Group,Chongqing 400038,China) |
| Abstract: | Candida E-2,a lipopetide biosurfactant producing strain,was isolated from sludge of wastewater treatment factory of Yangzi Petroleum Co.Ltd.The optimized medium composition by the single-factor experiment and orthogonal experiment was obtained(g/L): beef extract 3.0,sucrose 2.0,yeast extract 0.25,KH2PO4 12.5,MgSO4 0.3,NaCl 1.5,CaCl2 0.05,urea 0.5,initial pH 7.0,and liquid paraffin 10%(V/V).The optimized medium condition were inoculum amount 0.12 g/L,fermentation volume 30 mL/200 mL,fermentation time 5 d.Und... |
| Keywords: | biosurfactant Candida fermentation optimization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |