| L-谷氨酸补料分批发酵的研究 |
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| 引用本文： | 卢宗梅,康传利,张伟国.L-谷氨酸补料分批发酵的研究[J].生物加工过程,2011,9(5):27-31. |
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| 作者姓名： | 卢宗梅 康传利 张伟国 |
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| 作者单位： | 1. 中粮生物化学(安徽)股份有限公司,蚌埠,233010
2. 江南大学,工业生物技术教育部重点实验室,无锡,214122 |
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| 基金项目： | 国家高技术研究发展计划(863计划)资助项目 |
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| 摘 要： | 对谷氨酸棒杆菌(Corynebacteriuin glutamicum)HCJ46产L-谷氨酸的补料分批发酵条件进行研究.结果表明:最适初糖质量浓度和最佳残糖维持质量浓度分别为100和(10～20)g/L;对发酵控温方式进行研究,确定了最佳温度控制策略为0～8h维持32℃,8～16h维持34℃、16～32h维持36℃,同时发现相对溶氧控制在30%左右时产酸最高.在以上的优化条件下,L-谷氨酸产量从72g/L提高到95g/L,提高了31.9%.
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| 关 键 词： | 溶氧 分批补料 L-谷氛酸 温度 |
Study on fed-batch fermentation conditons of L-glutamic acid |
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| LU Zongmei,KANG Chuanli,ZHANG Weiguo.Study on fed-batch fermentation conditons of L-glutamic acid[J].Chinese Journal of Bioprocess Engineering,2011,9(5):27-31. |
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| Authors: | LU Zongmei KANG Chuanli ZHANG Weiguo |
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| Institution: | LU Zongmei1,KANG Chuanli2,ZHANG Weiguo2(1.COFCO Biochemical(Anhui) Co.,Ltd.,Bengbu 233010,China,2.Key Laboratory of Industrial Biotechnology of Ministry of Education,Jiangnan University,Wuxi 214122,China) |
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| Abstract: | Fed-batch fermentation conditions of Corynebacterium ghttamicum HCJ46 for L-glutamic acid production were studied, It was found that the optimal initial glucose concentration and maintained residual sugar concentration were 100 g/L, and 10 -20 g/L, respectively. The fermentation temperature control mode was further investigated. Results showed that the optimal control strategy was controlled as 0 - 8 h at 32 ℃, 8 - 16 h at 34℃, and 16 - 32 h at 36 ℃. It was also found that the highest glutamic acid productivity was obtained when the dissolved oxygen was controlled at 30%. As a result, under the optimization of fermentation conditions, L-glutamic acid productivity increased from 72 g/L to 95 g/L,improved by 29%. |
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| Keywords: | dissolved oxygen fed-batch L-glutamic acid temperature |
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