| 原生质体紫外诱变选育竹红菌甲素高产菌株 |
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| 引用本文： | 潘魏松,嵇源源,杨珠英,王剑文.原生质体紫外诱变选育竹红菌甲素高产菌株[J].生物加工过程,2012(6):18-23. |
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| 作者姓名： | 潘魏松 嵇源源 杨珠英 王剑文 |
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| 作者单位： | 苏州大学药学院,苏州215123 |
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| 基金项目： | 国家教育部留学基金资助项目（K513201011）;苏州大学东吴学者计划资助项目（14317363）;江苏省六大人才高峰项目（Bu132802）;国家大学生创新性实验计划资助项目 |
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| 摘 要： | 采用原生质体紫外诱变技术选育竹红菌甲素高产菌株。结果表明:以竹黄菌Shiraia sp.S8为出发菌株,当使用混合酶系(5 mg/mL纤维素酶和10 mg/mL蜗牛酶)在30℃处理菌丝2 h,获得菌丝原生质体3.24×106个/mL。以竹黄菌原生质体在距离15 W紫外灯30 cm处照射诱导,获得诱变菌株C6。其竹红菌甲素产量达到28.1 mg/L,比原始出发菌株提高了53.7%,且遗传稳定,具有较高的医药与工业应用价值。
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| 关 键 词： | 竹黄菌 原生质体诱变 竹红菌素 选育 |
Screening of high-yield hypocrellin A producing mutants from Shiraia sp. S8 by protoplast mutagenesis and ultraviolet irradiation |
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| PAN Weisong,JI Yuanyuan,YANG Zhuying,WANG Jianwen.Screening of high-yield hypocrellin A producing mutants from Shiraia sp. S8 by protoplast mutagenesis and ultraviolet irradiation[J].Chinese Journal of Bioprocess Engineering,2012(6):18-23. |
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| Authors: | PAN Weisong JI Yuanyuan YANG Zhuying WANG Jianwen |
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| Institution: | (College of Pharmaceutical Sciences, Soochow University,Suzhou 215123, China) |
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| Abstract: | A Shiraia sp. S8 mutant strain with higher yield of hypocrellin A was screened. Then, optimum conditions for protoplast preparation （3.24×10^6 mL-1） were determined. After 50 h cultivation the hy-pha were collected and digested with the mixing enzymes including cellulase （5 mg/mL） and snailase （ 10 mg/mL） for 2 h at 30℃. The C6 mutant with higher hypocrellin A yield was obtained via ultraviolet irradiation （30 cm from a 15 W UV lamp） on the isolated protoplasts. The yield of hyprocrellin A （28.1 mg/L） by mutant C6 was increased by 53.7% than that of the start strain S8. The production of the mu- tant was verified to be genetically stable. |
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| Keywords: | Shiraia sp protoplast mutation hypocrellin A screening |
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