| 响应面法优化甘蔗糖蜜发酵产丁醇 |
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| 引用本文： | 汤艳,郭亭,杜腾飞,张秋妍,陈啸鹏,姜岷.响应面法优化甘蔗糖蜜发酵产丁醇[J].生物加工过程,2012,10(5):7-12. |
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| 作者姓名： | 汤艳 郭亭 杜腾飞 张秋妍 陈啸鹏 姜岷 |
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| 作者单位： | 1. 南京工业大学生物与制药工程学院,南京,211800
2. 南京工业大学生物与制药工程学院,南京211800;广州甘蔗糖业研究所,广州510316 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)资助项目(2011CB707405);材料化学工程国家重点实验室基金资助项目(ZK201001);林业公益性专项基金资助项目(201004001);国家自然科学基金青年基金资助项目(21106067);江苏省高校自然科学研究重大项目(11KJA530001) |
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| 摘 要： | 以甘蔗糖蜜为底物,用响应面法对高丁醇比突变菌株拜氏梭菌(Clostridium beijerinckii)ART124发酵生产丁醇的培养条件进行优化.首先利用Plackett - Burman试验设计筛选出影响丁醇生产的3个重要因素CaCO3和NH4 HCO3和K2HPO4的用量,再通过最陡爬坡路径逼近最大向应区域,最后根据响应面中心组合设计理论,确定主要影响因素的最佳条件:CaCO3、NH4HCO3和K2HPO4的质量浓度分别为2.65、2.16和0.43 g/L.利用数学模型分析预测得甘蔗糖蜜质量浓度为30 g/L时,最佳的丁醇产量为8.10 g/L,比优化前提高了53.14％.在最佳工艺条件下得到的实验结果与模型预测值很吻合,说明所建立的模型是有效的.
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| 关 键 词： | Clostridium beijerinckii 丁醇 糖蜜 优化 |
Optimization of butanol fermentation with cane molasses by response surface method |
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| TANG Yan
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GUO Ting
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DU Tengfei
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ZHANG Qiuyan
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CHEN Xiaopeng
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JIANG Min.Optimization of butanol fermentation with cane molasses by response surface method[J].Chinese Journal of Bioprocess Engineering,2012,10(5):7-12. |
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| Authors: | TANG Yan GUO Ting DU Tengfei ZHANG Qiuyan CHEN Xiaopeng JIANG Min |
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| Institution: | 1(1.College of Biotechnology and Pharmaceutical Engineering,Nanjing University of Technology,Nanjing 211800,China; 2.Guangzhou Sugarcane Industry Research Institute,Guangzhou 510316,China) |
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| Abstract: | The butanol fermentation conditions of Clostridium beijerinckii ART124 from sugar cane molasses were optimized by response surface method.Plackett-Burman design was used to evaluate the influences of related factors.CaCO3,NH4HCO3 and K2HPO4 were selected as the significant factors.The path of steepest ascent was undertaken to approach the optimum region of the three significant factors.The effects of the three main factors on the butanol production were further investigated by a central composite design.The obtained optimal concentrations of nutrients were 2.65 g/L(CaCO3),2.16 g/L(NH4HCO3) and 0.43 g/L(K2HPO4).A corresponding mathematical model was established to predict the fermentation experiment and the maximum butanol yield of 8.10 g/L was obtained.Under the optimal conditions,the butanol concentration increased by 53.14%.The statistical method was proved to be a powerful tool for the optimization of butanol production from cane molasses. |
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| Keywords: | Clostridium beijerinckii butanol molasses optimization |
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