聚磷茵JPA1菌株的生长特性和聚磷条件的优化

以YG培养基为基础，考察不同条件对聚磷菌JPA1菌株生长及聚磷效率的影响。结果表明：JPA1菌株生长的对数期为3-9h，9h后进入稳定期，12h到达衰亡期。在单因素试验中，麦芽糖为JPA1菌株最适生长和聚磷的C源，最适生长温度为30-35℃，pH为8，Na＋、Ca2＋、Mg2＋、Mn2＋和K＋等离子有利于JPA1菌株的生长；最适聚磷温度为30-35℃，pH为7，Fe2＋、Na2＋、Mg2＋、Mn“和K＋等离子有利于JPA1菌株的聚磷。正交试验结果表明：JPA1菌株去磷培养基的最优组合为麦芽糖1．5g／L，温度37℃，pH6．5，装液量50mL（300mL摇瓶）；菌株最适扩增培养基为麦芽糖1．5g/L，温度37℃，pH6．5或7．5，装液量150mL（300mL摇瓶）。

Growth characteristics of phosphorus accumulating organism JPA1 strain and optimization of phosphorus accumulation condition

Based on the YG media, effects of different conditions to the growth and phosphorus accumula- tion rate of JPA1 strain were discussed. Result showed that the logarithmic phase of JPA1 was from 3 h to 9 h, it turned into stationary phase after 9 h, and then entered the state of death phase. Single factor ex- periment showed that maltose was the optimal carbon source for JPA1. The optimal growth temperature was 30 ℃ to 35 ℃. The best pH was 8. Metal ions of Na ＋ , Ca2 ＋ , Mg2 ＋ , Mn2 ＋ , and K ＋ were benefical to the growth of JPA1. The optimal phosphorus accumulation temperature was among 30 ℃ to 35 ℃, op- timal phosphorus accumulation pH was 7, Metal ions of Fe2＋ , Na ＋ , Mg2＋ , Mn2＋ , and K ＋ were good for phosphorus accumulation. Based on the YG media, using maltose to replace glucose, orthogonal experi- ment results showed that the best phosphorus accumulation condition was maltose 1.5 g/L, 37 ℃, pH 6.5, volume of liquid 50 mL （ shake flask culture in 300 mL） ; the best condition of culture was maltose 1.5 g/L, 37 ℃, pH 6.5 or 7.5, volume of 150 mL （shake flask culture in 300 mL）.