| NH4+浓度对黄色短杆菌XV0505发酵生产L-缬氨酸的影响 |
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| 引用本文： | 冯宁,白亚磊,徐庆阳,谢希贤,陈宁.NH4+浓度对黄色短杆菌XV0505发酵生产L-缬氨酸的影响[J].生物加工过程,2011,9(2):12-17. |
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| 作者姓名： | 冯宁 白亚磊 徐庆阳 谢希贤 陈宁 |
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| 作者单位： | 天津科技大学,生物工程学院,天津,300457 |
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| 基金项目： | 国家科技重大专项课题资助项目(2008ZX09401-05); 天津市科技支撑计划重点项目(08ZCKFSH01900); 天津市应用基础及前沿技术研究计划(08JCZDJC15400) |
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| 摘 要： | 以L-缬氨酸(L-Val)生产菌黄色短杆菌XV0505为供试菌株,以(NH4)2SO4为唯一添加N源,考察不同NH 4+浓度对发酵过程中菌体干质量、L-Val产量和葡萄糖消耗速率以及菌体内代谢流量的影响。研究表明:NH 4+浓度过高或不足都会影响发酵水平,降低L-Val的产量。合适的初始NH4+浓度为225 mmol/L,产酸期NH4+维持浓度为35 mmol/L时,有利菌体产酸。在此NH4+浓度下,在30 L发酵罐发酵60 h,发酵液中菌体生物量和L-Val质量浓度分别可达22.35和59.12 g/L。
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| 关 键 词： | 黄色短杆菌 代谢流 NH4 L-缬氨酸 |
Effect of NH4+concentration on L-valine fermentation by Brevibacterium flavum XV0505 |
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| FENG Ning,BAI Yalei,XU Qingyang,XIE Xixian,CHEN Ning.Effect of NH4+concentration on L-valine fermentation by Brevibacterium flavum XV0505[J].Chinese Journal of Bioprocess Engineering,2011,9(2):12-17. |
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| Authors: | FENG Ning BAI Yalei XU Qingyang XIE Xixian CHEN Ning |
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| Institution: | FENG Ning,BAI Yalei,XU Qingyang,XIE Xixian,CHEN Ning(College of Biotechnology,Tianjin University of Science & Technology,Tianjin 300457,China) |
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| Abstract: | The ammonium sulfate was selected as the only feeding nitrogen source in the L-valine fermentation process by Brevibacterium flavum XV0505.Effects of different NH+4 concentrations on biomass,yield of L-valine,glucose consumption rate and the metabolic flux were studied in a 30 L fermentor.Results showed that excessive or insufficient concentrations of NH+4 would adversely affect the yield of L-valine and bacterial growth.Therefore,the appropriate additive dosage of NH+4(225 mmol/L) was determined,and L-vali... |
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| Keywords: | Brevibacterium flavum metabolic flux NH 4 L-valine |
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