| 啤酒废酵母中β-1，3-葡聚糖的提取工艺 |
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| 引用本文： | 马森,卢家炯,林金梅,赵迎春,韦宇.啤酒废酵母中β-1，3-葡聚糖的提取工艺[J].生物加工过程,2009,7(5):39-43. |
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| 作者姓名： | 马森 卢家炯 林金梅 赵迎春 韦宇 |
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| 作者单位： | 广西大学,糖业工程技术中心,南宁,530004;广西大学,轻工与食品工程学院,南宁,530004 |
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| 基金项目： | 广西重大科技成果引进与产业化示范资助项目 |
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| 摘 要： | 研究采用酶-碱法从经超声波处理的废酵母残渣中提取β-1，3-葡聚糖的工艺，通过正交试验得出理想的酶处理工艺条件：酶添加量208U／g，温度50℃、pH6，酶解8h，蛋白质去除率为62．82％，每L废酵母液中可回收0．348g多肽、氨基酸的蛋白水解液；碱处理工艺条件：用30mL质量分数为2％ NaOH溶液在70℃处理酶解后的沉淀物5h。所得β-1，3-葡聚糖纯度为90．50％，得率为11．00％，经紫外光谱、薄层层析和性质分析为高纯度的β-1，3-葡聚糖。
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| 关 键 词： | 提取 β-1 3-葡聚糖 啤酒废酵母 |
Extraction of β-1,3-glucan from beer waste yeast |
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| MA Sen,LU Jia-jiong,LIN Jin-mei,ZHAO Ying-chun,WEI Yu.Extraction of β-1,3-glucan from beer waste yeast[J].Chinese Journal of Bioprocess Engineering,2009,7(5):39-43. |
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| Authors: | MA Sen LU Jia-jiong LIN Jin-mei ZHAO Ying-chun WEI Yu |
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| Institution: | 1. Research Center of Sugar Engineering, Guangxi University, Nanning 530004, China; 2. College of Light Industry and Food Engineering, Guangxi University, Nanning 530004, China) |
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| Abstract: | The enzyme-alkali extracting method for the β-1,3-glucan from beer waste yeast treated by ultrasonic waves was studied. Papain enzyme of 208 U/g substrate was added in the beer yeast and incubated for 8 h at pH 6 and 50 ℃. Protein removal rate was 62.82%. Protein hydrolysate （0. 348 g/L） containing po lypeptides and amino acids was recycled. The precipitate was treated with 30 mL 2% NaOH and water-bathed 5 h at 70 ℃. The yield of r-1,3-glucan was 11.00% by purity of 90.50%. |
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| Keywords: | extraction β-1 3-glucan waste beer yeast |
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