| 响应面法优化獐牙菜苦苷生物转化条件 |
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| 引用本文： | 常军,胡娜,张铁军,刘昌孝,田成旺.响应面法优化獐牙菜苦苷生物转化条件[J].生物加工过程,2010,8(1):28-33. |
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| 作者姓名： | 常军 胡娜 张铁军 刘昌孝 田成旺 |
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| 作者单位： | 1. 江西科技师范学院,南昌市天然产物与活性物质重点实验室,南昌,330013
2. 天津药物研究院,天津,300193 |
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| 基金项目： | 天津市应用基础重点资助项目，江西科技师范学院博士基金 |
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| 摘 要： | 采用响应面法对獐牙菜苦苷的生物转化条件进行优化,首先应用Plackett-Burman方法筛选出对转化具有显著性影响的因素,再用中心响应面法的Response Optimizer工具对最优转化条件进行了分析和预测,最后对预测结果进行了验证。结果表明,獐牙菜新碱的最优化转化条件(mg/mL)为MgSO4.7H2O 5.14、MnSO4.4H2O 3.42、葡萄糖9.95、蛋白胨9.24、pH5.80。在此条件下獐牙菜新碱的最大转化产率为17.64%;红百金花内酯的最优转化条件(mg/mL)为MgSO4.7H2O 4.09、MnSO4.4H2O6.07、葡萄糖7.37、酵母膏7.37,pH5.73。在此条件下红百金花内酯的最大转化产率为8.81%。
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| 关 键 词： | 獐牙菜苦苷 生物转化 獐牙菜新碱 红百金花内酯 |
Biotransformation optimization of swertiamarin by response surface methodology |
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| CHANG Jun,HU Na,ZHANG Tie-jun,LIU Chang-xiao,TIAN Cheng-wang.Biotransformation optimization of swertiamarin by response surface methodology[J].Chinese Journal of Bioprocess Engineering,2010,8(1):28-33. |
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| Authors: | CHANG Jun HU Na ZHANG Tie-jun LIU Chang-xiao TIAN Cheng-wang |
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| Institution: | CHANG Jun1,HU Na1,ZHANG Tie-jun2,LIU Chang-xiao2,TIAN Cheng-wang2(1.Nanchang Key Liboratory of Natural Product , Bioactive Materials,Jiangxi Science , Technology Normal University,Nanchang 330013,China,2.Tianjin Institute of Pharmaceutical Research,Tianjin 300193,China) |
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| Abstract: | Biotransformation conditions of swertiamarin were optimized by response surface methodology.The Plackett-Burman method was firstly used to screen significant factors on the biotransformation.Central response surface method was used to analyze the biotransformation and the response optimizer tool was used to estimate the optimal biotransformation conditions of swertiamarin to alkaloid and erythrocentaurin,respectively.Finally,the optimal conditions were verified by fermentation test.The optimal condition for... |
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| Keywords: | alkaloid biotransformation erythrocentaurin swertiamarin |
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