| 一株乙二醇氧化酶菌株的筛选及催化特性的初步研究 |
| |
| 引用本文： | 何玉财,徐晓锋,潘雪鹤,巫淼鑫.一株乙二醇氧化酶菌株的筛选及催化特性的初步研究[J].生物加工过程,2013(6):29-33. |
| |
| 作者姓名： | 何玉财 徐晓锋 潘雪鹤 巫淼鑫 |
| |
| 作者单位： | [1]常州大学制药与生命科学学院生物工程研究室,常州213164 [2]华东理工大学生物反应器工程国家重点实验室,上海200237 |
| |
| 基金项目： | 国家自然科学基金（21102011）; 常州市生物医药科技专项（CE20115051） |
| |
| 摘 要： | 利用富集培养技术从土壤中筛选获得1株高活性二醇氧化活性菌株Brevibacterium sp.CCZU12-1。以Brevibacterium sp.CCZU12-1静息细胞为催化剂,最适催化反应温度、反应pH和金属离子添加量分别为30℃、6.5和Mn2＋0.1 mmol/L。在最佳条件下,转化200 mmol/L乙二醇24 h,羟基乙酸的产率为94.6%,分批补料乙二醇5批,羟基乙酸的累积浓度为972 mmol/L。
|
| 关 键 词： | 氧化 乙二醇 羟基乙酸 Brevibacterium |
Isolation and catalytic characteristics of ethylene glycol-oxidizing strain |
| |
| HE Yucai,XU Xiaofeng,PAN Xuehe,WU Miaoxin.Isolation and catalytic characteristics of ethylene glycol-oxidizing strain[J].Chinese Journal of Bioprocess Engineering,2013(6):29-33. |
| |
| Authors: | HE Yucai XU Xiaofeng PAN Xuehe WU Miaoxin |
| |
| Institution: | 1. Laboratory of Biochemical Engineering,College of Pharmaceutical and Life Sciences ,Changzhou University,Changzhou 213164,China; 2. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China) |
| |
| Abstract: | Brevibacterium sp. CCZU12-1 had been isolated from soil samples with high diol-oxidizing activity against ethylene glycol using the enrichment culture technique. Using the resting cells of Brevibacterium sp. CCZU12-1 as catalysts, the optimal reaction temperature, pH and metal ion additive for diol-oxidizing activity were 30 ℃, 6.5 and Mn2＋ （ 0. 1 mmol/L ）, respectively. After 24 h of biotransformation,the yield of glycolic acid from 200 mmol/L ethylene glycol was 94.6%. Using fed- batch method, a total of 972 mmol/L of glycolic acid had been accumulated in the reaction mixture after the 5th feed. |
| |
| Keywords: | oxidation ethylene glycol glycolic acid Brevibacterium |
| 本文献已被 维普 等数据库收录！ |
|
