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| 姬松茸胞内多糖碱提取工艺 | |
| 引用本文： | 丁重阳,张笑然,张梁,王玉红,张志才,石贵阳,章克昌.姬松茸胞内多糖碱提取工艺[J].生物加工过程,2008,6(5):21-26. |
| 作者姓名： | 丁重阳 张笑然 张梁 王玉红 张志才 石贵阳 章克昌 |
| 作者单位： | 1. 江南大学,工业生物技术教育部重点实验室,无锡,214122;江南大学,生物工程学院,无锡,214122 2. 江南大学,生物工程学院,无锡,214122 3. 江南大学,生物工程学院,无锡,214122;江苏大学,食品与生物工程学院,镇江,212013 |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | 为了进一步提高姬松茸胞内多糖得率，采用单因素实验和响应曲面法，对姬松茸菌丝体胞内多糖碱提取工艺进行了研究。结果表明优化的碱提取工艺为提取时间4．63h，碱液浓度1．03mol／L，提取温度60．90℃，液料比85．85mL／g，在此条件下胞内多糖的提取得率为（273．49±1．59）mg／g干菌体，明显高于水提取所得到的胞内多糖得率。这为姬松茸碱提胞内多糖理化性质、生理活性等方面进一步的研究提供切实可行的高效提取方法。 |
| 关 键 词： | 姬松茸 提取 多糖 响应曲面法 液体发酵 |
Alkaline extraction process of intracellular polysaccharides from Agaricus blazei Murill |
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| DING Chong-yang,ZHANG Xiao-ran,ZHANG Liang,WANG Yu-hong,ZHANG Zhi-cai,SHI Gui-yang,ZHANG Ke-chang.Alkaline extraction process of intracellular polysaccharides from Agaricus blazei Murill[J].Chinese Journal of Bioprocess Engineering,2008,6(5):21-26. | |
| Authors: | DING Chong-yang ZHANG Xiao-ran ZHANG Liang WANG Yu-hong ZHANG Zhi-cai SHI Gui-yang ZHANG Ke-chang |
| Institution: | DING Chong-yang, ZHANG Xiao-ran , ZHANG Liang, WANG Yu-hong, ZHANG Zhi-cai , SHI Gui-yang, ZHANG Ke-chang (1. Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, China; 2. School of Biotechnology, Jiangnau University, Wuxi 214122, China ; 3. School of Food Science and Biotechnology, Jiangsu University, Zhenjiang 212013, China) |
| Abstract: | Alkaline extracting of intracellular polysaccharides （IPS） from Agaricus blazei Murill was investigated using response surface methodology. The results showed that the optimal extracting process conditions are as follows： extraction time 4.63 h, base concentration 1.03 mol/L , temperature 60.90 ℃, liquid to solid ratio 85.85 mL/g. Under the optimal conditions, the extracting ratio of crude IPS from Agaricus blazei Murill was （273.49 ± 1.59） mg/g dry mycelium. The method is better than that of water extracting and futher study of the characteristics of IPS is effective. |
| Keywords: | Agaricus blazei Murrill extracting polysaceharide response surface methodology submerged culture |
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