帆布固定化脂肪酶及其性能的研究

研究了用高碘酸钠氧化帆布纤维，使其纤维衍生化成为醛基，与脂肪酶交联进行固定化的过程。通过醛基被交联程度来评价交联过程的优劣。首先对纤维的氧化过程进行了简单优化，进而通过反复交联法与酶蛋白交联。以大豆油和橄榄油水解作为固定化酶的性能评价指标。实验结果表明，通过采用反复交联的方法，可提高载体表面酶蛋白质量分数30％左右。酶活力平均达到4U／cm^2，其对温度、pH的耐受性相比游离酶均有不同程度提高。同时利用油脂在固定化酶过程对酶进行保护，使其对温度、pH等的耐受性进一步增强。在维持较高水解率条件下，可在温和条件下连续反应7批，反应半衰期达140h以上。

Immobilization of lipase on canvas fiber and its characterization

The methods of lipase immobilization with canvas fiber were discussed in detail, and the function of immobilization lipase was determined. The NaIO4 oxidation has been employed in the process of changing the adjacent hydroxyl to aldehyde group. It was showed that through repeat cross-link, the lipase density on the carrier surface was enhanced about 30%. The activity of the immobilized lipase was investigated in catalyzing hydrolysis Oliver oil and soybean oil. The reaction stability was compared with those of the dissolved lipase in catalyzing hydrolysis of soybean oil. Through immobilization, its reaction tolerance to temperature and pH had been raised in some degree. With the lipase protection measure during the cross-link process, the tolerance of lipase to pH, temperature was improved further. The hydrolysis rate was more than 85% in the first run, and after 7 runs of continual reactions under mild conditions, the activity kept 64% of the original. The half-life of the immobilized lipase was more than 140 h.