Expressed Sequence Tag Analysis of Kidney and Gill Tissues from Rainbow Trout (Oncorhynchus mykiss) Infected with Infectious Hematopoietic Necrosis Virus |
| |
| Authors: | Tomoya Kono Masahiro Sakai Scott E LaPatra |
| |
| Institution: | (1) United Graduate School of Agricultural Science, Kagoshima University, Korimoto, 1-21-24, Kagoshima 890-0065, Japan, JP;(2) Faculty of Agriculture, Miyazaki University, Miyazaki 889-21, Japan, JP;(3) Clear Springs Foods Inc., P.O. Box 712, Buhl, ID 83316, U.S.A., US |
| |
| Abstract: | Expressed sequence tags (ESTs) were obtained from the kidney and gill tissues of rainbow trout, Oncorhynchus mykiss, infected with infectious hematopoietic necrosis virus (IHNV). The results of single-pass sequencing of ESTs from 198 clones
(AU081027–AU081192) from kidney complementary DNA and 45 clones (AU081193–AU081236) from gill cDNA are reported herein. Sequences
of the cDNA clones were compared with sequences in the GenBank database. Fourteen clones (20%) appeared to be completely unknown
and may represent newly described genes, whereas 158 clones (80%) were identified on the basis of matches to sequences in
the database. Three of the unidentified sequences were isolated from both the kidney and the gill cDNA libraries. However,
no sequences were identical between kidney and gill clones.
Received December 7, 1999; accepted April 28, 2000. |
| |
| Keywords: | : rainbow trout kidney leukocytes expressed sequence tags infectious hematopoietic necrosis virus |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
