Surface Display of <Emphasis Type="Italic">Aeromonas hydrophila</Emphasis> GAPDH in Attenuated <Emphasis Type="Italic">Vibrio anguillarum</Emphasis> to Develop a Noval Multivalent Vector Vaccine |
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Authors: | Yan Zhao Qin Liu Xiuhua Wang Lingyun Zhou Qiyao Wang Yuanxing Zhang |
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Institution: | (1) State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China;(2) Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, 166071, China; |
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Abstract: | Displaying foreign antigens on the surface of attenuated or avirulent bacteria is an important strategy to develop live multivalent
vector vaccines. In our previous work, several efficient surface display systems have been established based on outer membrane
anchoring elements, which could successfully display heterologous proteins in attenuated Vibrio anguillarum. In this work, the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from pathogenic Aeromonas hydrophila LSA34 was fused to seven display systems and introduced into attenuated V. anguillarum strain MVAV6203 (AV) to get seven GAPDH-display strains. The strain AV/pN-gapA showed the best display efficacy of GAPDH
and was tested as the multivalent vaccine candidate. Further immune protection evaluation of AV/pN-gapA in turbot (Scophtalmus maximus) demonstrated that the attenuated V. anguillarum with surface-displayed GAPDH of A. hydrophila LSA34 effectively protected turbot from the infections of A. hydrophila and V. anguillarum and showed potential value for further multivalent vaccine development. |
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