Research note: Analysis of expressed sequence tags from the chrysophycean alga Ochromonas danica (Heterokontophyta) |
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| Authors: | Makoto Terauchi Atsushi Kato Chikako Nagasato Taizo Motomura |
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| Institution: | 1. Graduate School of Environmental Science, Hokkaido University, Sapporo 060‐0810,;2. Division of Biological Science, Graduate School of Science, Sapporo 060‐0810, and;3. Muroran Marine Station, Field Science Center for Northern Biosphere, Hokkaido University, Muroran 051‐0003, Japan |
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| Abstract: | A cDNA library was constructed from the chrysophycean alga, Ochromonas danica E. G. Pringsheim. 5′‐end sequencing of about 600 cDNA clones yielded 476 authentic expressed sequence tags (EST) of which 275 showed significant matches (E‐value <10?4) to sequences in a public database. The annotation of these ESTs was carried out to assess subcellular localization of the putative proteins using several internet‐accessible prediction programs for subcellular localization. These analyses revealed that putative plastid proteins in Ochromonas possess N‐terminal bipartite presequences with a conserved phenylalanine at the N‐terminus of the predicted transit peptide‐like domains, similar to other ‘red‐lineage’ secondary symbiotic organisms. The examination of sequences of 3′‐UTR revealed that, similarly to chlorophyte algae, UGUAA may represent a putative polyadenylation signal in O. danica. |
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| Keywords: | Chrysophyceae codon usage expressed sequence tag Heterokontophyta Kozac sequence N‐terminal presequence Ochromonas danica polyadenylation signal Stramenopiles |
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