Detection and identification of cancerous murine fibroblasts,transformed by murine sarcoma virus in culture,using Raman spectroscopy and advanced statistical methods |
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Authors: | A Salman E Shufan L Zeiri M Huleihel |
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Institution: | 1. Department of Physics, SCE — Shamoon College of Engineering, Beer-Sheva 84100, Israel;2. Department of Chemistry, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel;3. Department of Virology and Developmental Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel |
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Abstract: | BackgroundCancer is one of the leading worldwide causes of death. It may be induced by a variety of factors, including carcinogens, radiation, genetic factors, or DNA and RNA viruses. The early detection of cancer is critical for its successful therapy, which can result in complete recovery from some types of cancer.MethodsRaman spectroscopy has been widely used in medicine and biology. It is a noninvasive, nondestructive, and water-insensitive technique that can detect changes in cells and tissues that are caused by different disorders, such as cancer.In this study, Raman spectroscopy was used for the identification and characterization of murine fibroblast cell lines (NIH/3T3) and malignant fibroblast cells transformed by murine sarcoma virus (NIH-MuSV) cells.ResultsUsing principal component analysis and LDA it was possible to differentiate between the NIH/3T3 and NIH-MuSV cells with an 80–85% success rate based on their Raman shift spectra.ConclusionsThe best results for differentiation were achieved from spectra that were obtained from the rich membrane sites.General significanceBecause of its homogeneity and complete control of most factors affecting its growth, cell culture is a preferred model for the detection and identification of specific biomarkers related to cancer transformation or other cellular modifications. |
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Keywords: | Raman spectroscopy NIH/3T3 NIH-MuSV Statistical analysis |
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