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Affinity of low molecular weight fucoidan for P-selectin triggers its binding to activated human platelets |
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| Authors: | Laure Bachelet Isabelle Bertholon Damien Lavigne Roger Vassy Martine Jandrot-Perrus Frédéric Chaubet Didier Letourneur |
| Institution: | 1. Inserm, U698, Cardiovascular Bioengineering, CHU X. Bichat, University Paris 7, Paris, F-75877, France;2. Institut Galilée, University Paris 13, Villetaneuse, F-93430, France;3. CNRS, UMR 7033, School of Medicine, University Paris 13, Bobigny, F-93017, France |
| Abstract: | Background: P-selectin is an adhesion receptor expressed on activated platelets and endothelial cells. Its natural ligand, P-selectin glycoprotein ligand-1, is expressed on leucocytes and the P-selectin/PSGL-1 interaction is involved in leukocyte rolling. We have compared the interaction of P-selectin with several low molecular weight polysaccharides: fucoidan, heparin and dextran sulfate. |
| Keywords: | PSGL-1 P-selectin glycoprotein ligand-1 KD dissociation constant FITC fluorescein isothiocyanate LMW low molecular weight SLex Sialyl Lewis X SPR surface plasmon resonance ABTS 2 2&prime -azino-di-[3-ethylbenzthiazoline-6sulfonic acid] TRAP thrombin receptor-activated-peptide ADP adenosine diphosphate PC5 PE-Cy5 phycoerythrine-cyanine 5 CD62P P-selectin SELDI-TOF MS surface-enhanced laser desorption/ionization time-of-flight mass spectrometry RU resonance units GlyCAM-1 glycosylation-dependent cell adhesion molecule-1 |
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