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Electrophysiological evidence for the autoregulation of β-cell secretion by insulin |
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| Authors: | Caroline S Page Franz M Matschinsky Paule E Lacy Susan Conant |
| Institution: | Departments of Pharmacology and Pathology, Washington University School of Medicine, St. Louis, Mo U.S.A. |
| Abstract: | Electrophysiological studies of cultured rat pancreatic β-cells using intracellular microelectrodes show that exogenous insulin over the range of 0.1–10.0 μg/ml inhibits the electrical activity due to 27.8 mM glucose in a dose-related manner. This inhibitory effect is manifested by a mean increase of the membrane potential from about ?20 to ?30 mV and inhibition of the manner of cells impaled showing spike activity from 60 to less than 10%. The inhibitory influence of insulin is rapid occuring within 5 min for the highest level used. The results provide evidence for a negative feedback role of insulin in regulating its own release. |
| Keywords: | To whom reprint requests should be sent at the present address: Diabetes Research and Education Hospital University of Alabama Hospitals and Clinics 1808 7th Avenue South Birmingham Ala 35294 U S A |
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