Arginase,an s-phase enzyme in a human cell line |
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Authors: | Sven Skog Viola Eriksson Eva Eliasson |
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Institution: | The Wenner-Gren Institute, University of Stockholm, Norrtullsgatan 16, S-113 45 Stockholm, Sweden |
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Abstract: | Suspension cultures of ‘Chang liver’ cells were synchronized by preincubation in a glutamine-deficient medium or by thymidine blockade. Specific arginase activity varied in the synchronized cultures, being high when the number of S-phase cells was maximal. A relationship between high arginase activity and a high percentage of (S+G2) cells was also found when unsynchronized cells were separated by velocity sedimentation. The increase in arginase activity near the G1/S border was totally inhibited in the presence of cycloheximide. The rate of decrease in activity after addition of the drug indicated that the variations in the rate of synthesis of the enzyme, while the rate of degradation was more or less constant, corresponding to 4–6% per h. The role of arginase in cells lacking a urea cycle and the regulation of arginase activity in ‘Chang liver’ cells is discussed. |
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Keywords: | Arginase Growth phasel Cycloheximide Synchronization (Chang liver cell) |
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