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Molecular characterization and physico-chemical analysis of a new giant embryo mutant allele (ge t) in rice (Oryza sativa L.) |
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| Authors: | Dong-Soo Park Soo-Kwon Park Bong-Chun Lee Song-Yi Song Nam-Soo Jun Norvie L Manigbas Jun-Hyun Cho Min-Hee Nam Jong-Seong Jeon Chang-Deok Han Kyung-Jin Choi Doh-Hoon Kim Youngmin Woo Hee-Jong Koh Hang-Won Kang Gihwan Yi |
| Institution: | 1. Department of Functional Crop, National Institute of Crop Science, Rural Development Administration, 627-130, Milyang, Korea 2. National Institute of Crop Science, Rural Development Administration, 441-857, Suwon, Korea 3. Plant Metabolism Research Center & Graduate School of Biotechnology Kyung Hee University, 449-701, Yongin, Korea 4. Gyeongsang National University, Jinju, Korea 5. College of Natural Resources and Life Science, Dong-A University, 604-714, Busan, Korea 6. Department of Life Science, Pohang University of Science and Technology, 790-784, Pohang, Korea 7. College of Agricultural and Life Science, Seoul National University, Seoul, Korea |
| Abstract: | The rice embryo is rich in lipid and protein bodies, bioactive chemicals such as dietary fiber, phytic acids, vitamin B and E, and gamma aminobutyric acid (GABA) than the endosperm. In this paper, we report a new giant embryo mutant,ge t, induced from somaclonal variation derived by anther culture in rice. Sequence analysis of Cytochrome P450 of the get mutant revealed thatge t is a new allele of theGE gene with a single point mutation with substitution of amino acid, W395 to L395. The weight of thege t mutant embryo was 3.7 times higher than normal embryo. Tocopherol and mineral content were also higher than the previously reported giant embryo rice variety, Keunnun. These results indicated that this new giant embryo rice (ge t) offers a promising source of genetic material in improving nutritional quality of rice especially tocopherol, essential minerals, and GABA. |
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