ES cell neural differentiation reveals a substantial number of novel ESTs |
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Authors: | G Bain FC Mansergh MA Wride JE Hance A Isogawa SL Rancourt WJ Ray Y Yoshimura T Tsuzuki DI Gottlieb DE Rancourt |
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Institution: | (1) Department of Oncology, The University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta, Canada, T2N 4N1,;(2) Department of Biochemistry and Molecular Biology, The University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta, Canada, T2N 4N1,;(3) Department of Anatomy and Neurobiology, Washington University School of Medicine, 660 South Euclid Ave, St. Louis, MO 63110, USA,;(4) Department of Medical Biophysics and Radiation Biology, Graduate School of Medical Sciences, Kyushu University, 1–1, Maidashi 3-Chome, Higashi-Ku, Fukuoka, 812–8582, Japan,;(5) Present address: AVENTIS Pharmaceuticals Inc., 26 Landsdowne Street, Cambridge, MA 02139–4234, USA, |
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Abstract: | We have used a method for synchronously differentiating murine embryonic stem (ES) cells into functional neurons and glia
in culture. Using subtractive hybridization we isolated approximately 1200 cDNA clones from ES cell cultures at the neural
precursor stage of neural differentiation. Pilot studies indicated that this library is a good source of novel neuro-embryonic
cDNA clones. We therefore screened the entire library by single-pass sequencing. Characterization of 604 non-redundant cDNA
clones by BLAST revealed 96 novel expressed sequence tags (ESTs) and an additional 197 matching uncharacterized ESTs or genomic
clones derived from genome sequencing projects. With the exception of a handful of genes, whose functions are still unclear,
most of the 311 known genes identified in this screen are expressed in embryonic development and/or the nervous system. At
least 80 of these genes are implicated in disorders of differentiation, neural development and/or neural function. This study
provides an initial snapshot of gene expression during early neural differentiation of ES cell cultures. Given the recent
identification of human ES cells, further characterization of these novel and uncharacterized ESTs has the potential to identify
genes that may be important in nervous system development, physiology and disease.
Electronic Publication |
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Keywords: | Embryonic stem cell Expressed sequence tag Neural differentiation Gene discovery |
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