自噬基因PULK、PI3KC3在宫颈病变的表达及相关性研究

目的探讨自噬基因PULK、PI3KC3在正常宫颈组织及病变宫颈组织的表达及相关性。方法随机抽取正常宫颈组织30例、CIN20例及宫颈癌45例,采用qRT-PCR法检测组织中PULK和PI3KC3基因表达水平,免疫组织化学染色方法定性检测PULK、PI3KC3在正常宫颈组织及病变宫颈组织中的表达情况。结果免疫组化结果显示,PULK和PI3KC3在CIN及宫颈癌组织中的阳性表达显著低于正常宫颈组织,CIN及宫颈癌组织的PULK和PI3KC3表达水平均低于正常宫颈组织(P0.05或P0.01)。PULK与PI3KC3表达呈正相关(r=0.862,P0.01)。结论 PULK和PI3KC3可能参与宫颈病变的恶性转变过程。

Expression autophagy-related gene PULK and PI3KC3 and theircorrelation with cervical cancer

Objective To examine expression levels of autophagy gene PULK and PI3KC3 and to explore their correlation with cervical cancer . Methods A total of 95 samples of surgical resection from cervical specimens (including 30 cases of normal cervical tissue ,20 cases of cervical intraepithelial neoplasia ,and 45 cases of cervical carcinoma) were randomly selected .Expressions of pULK and PI3KC3 in cervical tissues were assessed by immu‐nohistochemistry and by real‐time quantitative PCR (qRT‐PCR) . Results The expression levels of PULK and PI3KC3 are significantly lower in patient with cervical intraepithelial neoplasia and cervical carcinoma than those in normal cervical tissue .Immunohistochemistry and qRT‐PCR analysis confirmed that PULK and PI3KC3 expres‐sions were significantly down‐regulated in patients with cervical intraepithelial neoplasia and cervical carcinoma . Correlation analysis showed that the expression of PI3KC3 was positively correlated with PULK . Conclusion PULK and PI3KC3 genes may be involved in the process of malignant transformation of cervical lesions .