| 尖吻蝮蛇毒中精氨酸脂酶的分离纯化与性质研究 |
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| 引用本文： | 郑颖,向左云,姚光宁,王维详,杨美峰.尖吻蝮蛇毒中精氨酸脂酶的分离纯化与性质研究[J].蛇志,2003,15(1):3-5. |
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| 作者姓名： | 郑颖 向左云 姚光宁 王维详 杨美峰 |
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| 作者单位： | 成都军区联勤部军事医学研究所,云南,昆明,650032 |
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| 摘 要： | 目的：从尖吻蝮蛇蛇毒中纯化出精氨酸脂酶并进行性质研究。方法：利用亲和层析、DEAE—Sepharose FF、Superdex 75柱对尖吻蝮蛇毒进行分离纯化。结果：分离纯化出的2个精氨酸脂酶活性组份分子量分别为36000、30000，它们的HPLC纯度也都达到97％以上。结论：为其临床应用打下了基础。
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| 关 键 词： | 尖吻蝮蛇毒 精氨酸脂酶 分离 纯化 性质 |
| 文章编号： | 1001-5639(2003)01-0003-03 |
Purification of Arginine-esterase from Agkistrodon acutus Snake Venom |
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| ZHENG Ying,XIANG Zuo Yun YAO Guang Ning,WANG Wei Xiang,YANG Mei Feng.Purification of Arginine-esterase from Agkistrodon acutus Snake Venom[J].Journal of Snake,2003,15(1):3-5. |
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| Authors: | ZHENG Ying XIANG Zuo Yun YAO Guang Ning WANG Wei Xiang YANG Mei Feng |
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| Abstract: | Objective Purificating Arginine esterase from Agkistrodon acutus venom Methods Using column chromatography separation consisting of affinity chromatography, DEAE Sepharose FF ion exchange and Superdex 75 PG gel filtration Result They are single band on SDS PAGE electrophoresis, and a single peck in HPLC They molecular weights were 36000, 30000 They are different on some properties Conclusion This study provided a basis for clinical application of Arginine esterase |
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| Keywords: | Arginine esterase Agkistrodon acutus venom purify |
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