王锦蛇血清对尖吻蝮蛇毒的抑制作用

目的 探讨王锦蛇血清对尖吻蝮蛇毒的抑制作用。方法 王锦蛇血清与不同剂量的尖吻蝮蛇毒分别混合后，注射到小鼠背皮下，测定王锦蛇血清对尖吻蝮蛇毒的抗出血活力；腹腔注射此混合物后，测定王锦蛇血清对尖吻蝮蛇毒的抗毒效价；先后注射尖吻蝮蛇毒和王锦蛇血清，测定王锦蛇血清对尖吻蝮蛇毒引起的死亡、组织损伤和炎症的抑制、保护和治疗作用。结果 1ml王锦蛇血清可完全抑制10mg（干重）的尖吻蝮蛇毒的出血活力；1ml王锦蛇血清可中和11mg（干重）尖吻蝮蛇毒的致死活力；王锦蛇血清对由尖吻蝮蛇毒引起的致死、组织损伤和炎症有显著的抑制、保护和治疗作用。结论 王锦蛇血清是尖吻蝮蛇毒的强抑制剂，可能成为未来新的蛇伤治疗药物的原料。

Inhibition of Elaphe carinata Serum against Deinagkistrodon acutus Venom

Objective To study the inhibitive effects of Elaphe carinata Serum ( ECS) against Deinagkistrodon acutus Venom ( DAV). Methods The certain amount of ECS and increasing doses of DAV were mixed in vitro. This mixture was injected into dorsal skin of mice to determine the anti-hemorrhagic activity of ECS against DAV, and injected ip to determine the neutralizing valence. In order to observe the inhibitive, protective and therapeutic effects of ECS on lethality, lesion and inflammation induced by DAV, we orderly injected DAV and ECS into mice and rats. Results 1ml ECS can completely inhibit the hemorrhagic activity of 10mg DAV (dry weight). 1ml ECS can neutralize the lethal activity of 11mg DAV (dry weight). It is very significant that the inhibitive, protective and therapeutic effects on lethality, lesion and inflammation induced by DAV. Conclusion ECS possesses potent anti-DAV activities and might be a possible source of new antivenom drug.