拔毒消炎软膏质量控制研究

目的研究拔毒消炎软膏的质量控制方法。方法用薄层色谱法(HPLC)对制剂中大黄、黄柏进行定性鉴别,高效液相色谱法测定大黄酚的含量。结果定性鉴别薄层色谱斑点特征明显;高效液相色谱法测定含量,大黄酚在0.061~0.304μg/ml范围内呈良好的线性关系(r=0.9954),平均加样回收率为98.68%,RSD=1.39%。结论采用薄层色谱法定性、高效液相色谱法定量,简便准确、重现性良好,可有效控制该制剂质量。

Study of Quality Control for Badu Xiaoyan Ointment

Objective To study the method of quality control for Badu Xiaoyan ointment . Methods The thin layer chromatography （TLC） was used to the qualitative identification of rheum officinale in the preparations .High Performance Liquid Chromatography （HPLC） was used to determine the content of Chrysophanol . Results The characteristic of TLC spots is obvious in the qualitative identification .HPLC determine the content .The Chry-sophanol in 0.061-0.304 μg/ml extent Show good linear relation（r=0.9954） .The recovery of mean sampling is 98.68% ,RSD=1.39% . Conclusion The present study employ TLC to quality and HPLC to quantify that were simple and accurate and has good repeatability .This may effective control the quality of xiaoyu zhitong ointment .