| 川鄂山茱萸不同部位中熊果酸的含量测定 |
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| 引用本文： | 王丽萍,宋良科,董关涛,李迪强.川鄂山茱萸不同部位中熊果酸的含量测定[J].中国野生植物资源,2009,28(5):60-62. |
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| 作者姓名： | 王丽萍 宋良科 董关涛 李迪强 |
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| 作者单位： | 1. 西南交通大学生命科学与工程学院,四川,成都,610031
2. 中国林业科学院生态所,北京,100091 |
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| 基金项目： | 自然保护区生物标本整理-整合共享试点平台建设项目- |
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| 摘 要： | 目的：测定川鄂山茱萸不同部位中熊果酸的含量,筛选含量最高的部位,为充分利用川鄂山茱萸野生资源提供依据。方法：采用SymmetrySheild TM RP18色谱柱4.6mm×250mm,5μm）;乙腈-水-磷酸（80∶20∶0.1）为流动相;检测波长为206nm;流速为1.0mL/min;柱温为30℃。结果：熊果酸在0.42mg～2.94mg范围内呈良好的线性关系（r=0.9998）,平均回收率为96.27%,RSD为1.4%。结论：川鄂山茱萸不同部位均含有熊果酸,且含量有明显差别。川鄂山茱萸总苞片的熊果酸含量为1.1044%,有一定的开发价值。
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| 关 键 词： | 熊果酸 川鄂山茱萸 高效液相色谱法 |
Dertermination the Content of Ursolic Acid in Cornus chinensis |
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| Wang Liping,Song Liangke,Dong Guantao,Li Diqiang.Dertermination the Content of Ursolic Acid in Cornus chinensis[J].Chinese Wild Plant Resources,2009,28(5):60-62. |
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| Authors: | Wang Liping Song Liangke Dong Guantao Li Diqiang |
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| Institution: | Wang Liping , Song Liangke , Dong Guantao, Li Diqiang (1. Life Science and Engineering College of Southwest Jiaotong University,Emei 610031,2. Research Institute of Ecology, China Academy of Forestry, Beijing 10091, China) |
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| Abstract: | Objective To determine the content of ursolie acid in different parts of Comus chinensis Wanger, and screen the content highest part in it. This provides the basis for the full use of wild resources. Method HPLC analysis was carried out using DJZ column （4.6 mm ×250 mm,5 μm） and acetonitril - water -phosphoric acid （80： 20：0.1 ） as the mobilephase. The detection wavelength was 206 nm, the flow rate was 1.0 ml/min, and the column temperature was 30℃. Result The linear range was 0. 42 mg- 2.94 mg（r =0.999 8）. The average recovery of the method was 96.27% （r = 1.4）. Conclusion The different parts of Comus chinensis Wanger imply the ursolie acid. The content is obviously different. The content of ursolie acid in the involucre of Comus chinensis was 1. 1044%, so it has some value to develop it. |
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| Keywords: | ursolie acid Cornus chinensis Wanger HPLC |
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