| 八角叶总黄酮的提取及其捕获自由基作用研究 |
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| 引用本文： | 莫丽玲,肖词英,黄锁义,黄世稳,黄丽秀,马连荣.八角叶总黄酮的提取及其捕获自由基作用研究[J].中国野生植物资源,2011,30(1):50-53. |
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| 作者姓名： | 莫丽玲 肖词英 黄锁义 黄世稳 黄丽秀 马连荣 |
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| 作者单位： | 1. 右江民族医学院医学检验学院,广西,百色,533000
2. 右江民族医学院药学系,广西,百色,533000 |
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| 基金项目： | 广西壮族自治区教育厅课题，广西壮族自治区百色市科学研究与技术开发计划第一批项目，右江民族医学院普高学生科研课题 |
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| 摘 要： | 目的:为八角叶资源的合理开发和利用提供科学依据。方法:分别以乙醇、石油醚、水等作提取溶剂,用超声波与非超声波对比提取八角叶总黄酮,考察在不同温度、溶剂浓度、超声波功率、提取时间等不同条件下用超声波/乙醇浸提法提取八角叶总黄酮,对所提取的黄酮类物质进行验证,并用分光光度法测定含量,用八角叶总黄酮对羟自由基清除作用进行试验。结果:超声波乙醇浸提法提取八角叶总黄酮的最佳工艺条件为:溶剂90%乙醇,温度80℃,超声波功率60 W,提取时间3.0 h。百色、南宁、贺州、河池、钦州所产八角叶总黄酮的含量分别为0.1649mg/mL、0.1022 mg/mL、0.1122 mg/mL、0.1850 mg/mL、0.1693 mg/mL,八角叶总黄酮提取液对Fenton体系产生的.OH自由基有很好的清除作用。结论:超声波乙醇浸提法提取八角叶总黄酮的效果最佳,产总黄酮含量最高,提示八角叶具有较高的开发利用价值。
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| 关 键 词： | 八角叶 总黄酮 提取工艺 含量测定 自由基 清除 |
Extraction and Free Radical Scavenging Investigation of Total Flavonones from Anise Leaves |
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| Mo Liling,Xiao Ciying,Huang Suoyi,Huang Shiwen,Huang Lixiu,Ma Lianrong.Extraction and Free Radical Scavenging Investigation of Total Flavonones from Anise Leaves[J].Chinese Wild Plant Resources,2011,30(1):50-53. |
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| Authors: | Mo Liling Xiao Ciying Huang Suoyi Huang Shiwen Huang Lixiu Ma Lianrong |
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| Institution: | Mo Liling1,Xiao Ciying1,Huang Suoyi2,Huang Shiwen2,Huang Lixiu1,Ma Lianrong1(1.College of Laboratory Medicine,Youjiang Medical College for Nationalities,Baise 533000,China,2.Department of Pharmacy,China) |
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| Abstract: | OBJECTIVE In order to provide a scientific foundation for the rational development and utilization of anise leaves.METHODS Respectively sherwood oil,ethanol and water for use as solvent extraction,extracting of total flavonones from the anise leaves by comparing ultrasonic wave with non-ultrasonic wave.Studying the method of extracting total flavonones from anise leaves under different conditions,such as different temperatures,solvent concentration,ultrasonic power,verifying the total flavonones and using v... |
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| Keywords: | anise leaves total flavonones extraction process definite quantity hydroxyl radical scavenging |
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