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| 新药23-羟基白桦酸的含量及其他三萜的RP-HPLC测定 | |
| 引用本文： | 王勇,范春林,王英,叶文才.新药23-羟基白桦酸的含量及其他三萜的RP-HPLC测定[J].中国野生植物资源,2011,30(4):50-52. |
| 作者姓名： | 王勇 范春林 王英 叶文才 |
| 作者单位： | 1. 江苏省食品药品检验所,江苏南京210008;中国药科大学天然药化教研室,江苏南京210009 2. 暨南大学中药及天然药物研究所,广东广州,510632 3. 暨南大学中药及天然药物研究所,广东广州510632;中国药科大学天然药化教研室,江苏南京210009 |
| 基金项目： | 广东省关键领域重大突破项目(2003A30909); 广州市科技计划项目(2004Z2-E5021) |
| 摘 要： | 目的:建立23-羟基白桦酸及有关物质的RP-HPLC测定方法。方法:高效液相色谱法,色谱柱为Agilent Zorbax SB-C18(250 mm×4.60 mm,5μm),流动相:乙腈-0.2%醋酸水溶液(62∶38),流速为0.8 mL.min-1;检测波长204 nm。结果:23-羟基白桦酸进样量与峰面积呈良好线性关系,线性范围为0.20~4.0μg,r=0.999 9(n=5);回收率为97.4%~104.7%(n=9)。结论:本法快速,简便,重复性好,可用于23-羟基白桦酸的含量测定和有关物质检查。 |
| 关 键 词： | 23-羟基白桦酸 有关物质 反相高效液相色谱法 |
Determination of 23-hydroxylbetulinic Acid and Its Related Substances by RP-HPLC |
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| Wang Yong , Fan Chunlin , Wang Ying , Ye Wencai.Determination of 23-hydroxylbetulinic Acid and Its Related Substances by RP-HPLC[J].Chinese Wild Plant Resources,2011,30(4):50-52. | |
| Authors: | Wang Yong Fan Chunlin Wang Ying Ye Wencai |
| Institution: | Wang Yong1,Fan Chunlin2,Wang Ying2,Ye Wencai2,3(1.Jiangsu Institute of Food and Drug Control,Nanjing 210009,China,2.Institute of Traditional Chinese Medicine & Natural Products,Jinan University,Guangzhou 510632,China 3.Department of Phytochemistry,China Pharmaceutical University,China) |
| Abstract: | Objective： To establish an HPLC method to determine the content of 23-hydroxylbetulinic acid and its related substances.Methods： An HPLC method was developed.A Zorbax SB-C18 column was used,and the mobile phase consisted of acetonitrile and 0.2% acetic acid solution（62：38）.Flow rate was 0.8 mL·min^-1.Detection was at 204 nm.Results：The linear range was 0.20～4.0 μg,r = 0.999 9（n=5）.The average recovery was 101.9%～104.7%（n=9）.Conclusion： This reliable and reproducible HPLC analysis method offers an option in quality control of 23-hydroxylbetulinic acid. |
| Keywords: | 23-hydroxylbetulinic acid related substances RP-HPLC |
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