| 蛇床子有效成分的RP—HPLC测定及其4种提取方法的比较研究 |
| |
| 引用本文： | 吴芳,刘新宇,范国荣,胡晋红.蛇床子有效成分的RP—HPLC测定及其4种提取方法的比较研究[J].中国野生植物资源,2001,20(6):52-54. |
| |
| 作者姓名： | 吴芳 刘新宇 范国荣 胡晋红 |
| |
| 作者单位： | 1. 广东医学院附属福田医院,药剂科,深圳,518033
2. 第二军医大学,上海,200433 |
| |
| 基金项目： | 本课题为国家自然科学基金(39770905)资助项目 |
| |
| 摘 要： | 以蛇床子素、欧前胡素、佛手柑内酯为指标成分，建立了蛇床子有效成分分离测定的HPLC新方法，并对95%乙醇冷浸、超声、回流、索氏提取蛇床子所得的香豆素成分进行HPLC分析与比较。结果表明，HPLC新方法适合于蛇床子成分的质量控制，其中蛇床子不同提取液中各香豆素成分含量测定差异较大，且以超声提取为最佳。
|
| 关 键 词： | 蛇床子 蛇床子素 欧前胡素 佛手柑内酯 RP－HPLC 提取方法 药用成分 |
Determination and Comparison of Coumarins in Four Kinds of Fructus Cnidii Extract Solutions |
| |
| Wu Fang ,Liu Xinyu ,Fan Guorong ,Hu Jinhong.Determination and Comparison of Coumarins in Four Kinds of Fructus Cnidii Extract Solutions[J].Chinese Wild Plant Resources,2001,20(6):52-54. |
| |
| Authors: | Wu Fang Liu Xinyu Fan Guorong Hu Jinhong |
| |
| Institution: | Wu Fang 1,Liu Xinyu 1,Fan Guorong 2,Hu Jinhong 2 1.Futian Hospital,Guangdong Medical College,Shenzhen 518033, 2. Second Military Medical University,Shanghai 200433 ) |
| |
| Abstract: | Selecting osthol, imperatorin and bergapten as the indexes, a new HPLC method was developed and the extraction methods of maceration, ultrasound, reflux and Soxhlet's apparatus with 95% ethanol were compared, and the coumarins in four kinds of fructus cnidii extract solutions were determined by HPLC. The experimental results showed that the new HPLC method was suitable for the quality control of fructus cnidii extracts and the ultrasound is the best method for the coumarins extraction in fructus cnidii. |
| |
| Keywords: | fructus cnidii osthol imperatorin bergapten RP-HPLC extraction method |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
