| RP-HPLC法测定泽兰中桦木酸的含量 |
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| 引用本文： | 李超,钱士辉.RP-HPLC法测定泽兰中桦木酸的含量[J].中国野生植物资源,2013(6):42-44. |
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| 作者姓名： | 李超 钱士辉 |
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| 作者单位： | 南京中医药大学江苏南京210046;江苏省中医药研究院,江苏南京210028 |
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| 基金项目： | 江苏省重大科技项目（BM2010610） |
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| 摘 要： | 目的：建立反相高效液相色潽法测定泽兰中桦木酸的含量.方法：采用AlltimaTM-C18（250 mm×4.6 mm,5μm）;以甲醇-0.2％磷酸水溶液=85：15进行等度洗脱,流速为1.0 mL/min,检测波长为202 nm,柱温为35℃.结果：桦木酸的线性范围为3.968～99.200 μg（r=0.997 3）,平均回收率为100.87％.结论：该方法结果准确、简便可行、重复性好,可为泽兰的质量控制提供依据.
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| 关 键 词： | 泽兰 桦木酸 反相液相色谱法 含量测定 |
Determination of Betulinic Acid in Lycopi Herba by HPLC |
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| Li Chao,Qian Shihui.Determination of Betulinic Acid in Lycopi Herba by HPLC[J].Chinese Wild Plant Resources,2013(6):42-44. |
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| Authors: | Li Chao Qian Shihui |
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| Institution: | 1. Nanjing University of Chinese Medicine, Nanjing 210046, China; 2. Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China) |
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| Abstract: | Objective： To establish a method for determination of betulinic acid by HPLC. Methods： A- nalysis was carried out on a AlltimaTM -C18（250 mm ×4.6 mm, 5 μm） eluted with water solution con- taining 0.2% phosphoric acid-methanol as the mobile phase in isocratic elution at the flow rate of 1.0 mL mm . The UV detection wavelength was 202 nm, the temperature of column was 35 ℃. Results： The linear response range was within 3. 968 - 99. 200 μg （ r = 0. 997 3, n = 5 ）. The recovery of it was 100.87%. Conclusion： This method is simple, rapid and accurante, particularly suitable for the quality control of Lycopi Herba. |
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| Keywords: | Lycopi Herba betulinic acid RP-HPLC determination |
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