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绞股蓝茎尖组织培养和植株再生研究
引用本文:刘松青,武成荣.绞股蓝茎尖组织培养和植株再生研究[J].中国野生植物资源,2000,19(6):60-61.
作者姓名:刘松青  武成荣
作者单位:[1]四川省喜德米市中学生物工程技术研究所 [2]四川师范大学化学与生命科学学院,成都
摘    要:以绞股蓝茎尖作为外植体,接种于MS+1mg/L BA+0.1mg/L NAA的培养基中,茎尖逐渐形成芽苗,芽苗分离后接种于MS+1mg/L BA+0.1mg/L NAA的培养基中进行增殖培养,同时从增殖的芽苗上不断地诱导出幼苗,将幼苗分离转入不含激素的MS培养基中,幼苗生根,形成完整的植株。

关 键 词:绞股蓝  茎尖  组织培养  植株再生

Shoot Tips Culture and Plants Regeneration in Gynostemma pentaphyllum(Thunb.)Makino in vitro
Liu Songqing.Shoot Tips Culture and Plants Regeneration in Gynostemma pentaphyllum(Thunb.)Makino in vitro[J].Chinese Wild Plant Resources,2000,19(6):60-61.
Authors:Liu Songqing
Abstract:Shoot tips in Gynostemma pentaphyllum(Thunb.)Makino cultured on Murashige and Skoog basal salts medium supplemented with 1mg/L BA and 0.1mg/L NAA and buds from Shoot tips was observed.Bud seedlings were transferred to MS medium supplemented with 1mg/L BA and 0.1mg/L NAA and those were produced new seedlings.When the rootless seedlings transfered on MS medium supplemented not exogenous hormones plantlets with roots would be gotten.
Keywords:Gynostemma pentaphyllum(Thunb  )Makino  Shoot tips  Tissue Culture  Plant Regenerationh
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