| 基于RAPD技术探讨黄精属部分药用植物系统位置 |
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| 引用本文： | 朱艳,孙伟,秦民坚,张朝凤.基于RAPD技术探讨黄精属部分药用植物系统位置[J].中国野生植物资源,2011,30(6):34-37. |
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| 作者姓名： | 朱艳 孙伟 秦民坚 张朝凤 |
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| 作者单位： | 1. 中国药科大学中药学院,江苏南京,211198
2. 无限极(中国)有限公司,广东广州,510665 |
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| 摘 要： | 目的：从分子水平探讨黄精属部分药用植物间的系统位置。方法：采用RAPD分子标记方法，以同科万寿竹属植物宝铎草（Disporum sessile D．Don）为外类群，对百合科黄精属19批（6种）药用植物进行基因组DNA的多态性分析，通过聚类分析探讨黄精种属间亲缘关系。结果：筛选出7条随机引物，聚类结果表明，黄精属植物在属级分类上特征明显，但属内分类有交叉。结论：RAPD技术可以为黄精属的分类和种的鉴别提供一定的理论依据。
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| 关 键 词： | 黄精属 RAPD 聚类分析 系统位置 |
Systematic Position of Some Medicinal Plants from Polygonatum Based on RAPD |
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| Zhu Yan,Sun Wei,Qin Minjian,Zhang Chaofeng.Systematic Position of Some Medicinal Plants from Polygonatum Based on RAPD[J].Chinese Wild Plant Resources,2011,30(6):34-37. |
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| Authors: | Zhu Yan Sun Wei Qin Minjian Zhang Chaofeng |
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| Institution: | 1. Department of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing 211198, China ;2. Unlimited Extremely China Co, Guangzhou 510665, China) |
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| Abstract: | Objective： To study the systematic position of some medicinal plants from genus Polygonatum through molecular technology. Methods： The DNA polymorphisms of 19 groups （6 species ） from genus Polygonatum and Disporum sessile D. Don as the outgroup were detected by RAPD molecular markers. Results： Seven arbitrary primers were screened for the RAPD analysis, phylogenetic trees revealed that the classified features of genus were obvious while the classification of inter - genus was crossed. Conclusion： RAPD markers may offer some evidence for the identification and categorization from genus Polygonatum. |
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| Keywords: | Polygonatum RAPD culster analysis systematic position |
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