首页 | 本学科首页   官方微博 | 高级检索  
   检索      

天胡荽属药用植物的积雪草苷与羟基积雪草苷的含量测定
引用本文:董关涛,宋良科,汤昊,王岩,王小宁.天胡荽属药用植物的积雪草苷与羟基积雪草苷的含量测定[J].中国野生植物资源,2012,31(2):47-50.
作者姓名:董关涛  宋良科  汤昊  王岩  王小宁
作者单位:西南交通大学 生命科学与工程学院,四川成都,610031
基金项目:西南交通大学基金(2006B10);国家自然保护区生物标本-民族医药标本整理整合(2005DKA21404)
摘    要:目的:建立天胡荽属植物的积雪草苷和羟基积雪草苷HPLC含量分析的方法,为开发利用天胡荽属植物药用资源奠定基础。方法:以积雪草苷和羟基积雪草苷为指标成分,采用高效液相色谱法,以Symmetry C18(4.6 mm×250 mm,5μm)为色谱柱;乙腈-水(29∶71,v/v)为流动相;流速0.8 mL/min;柱温30℃;检测波长210 nm,外标法进行测定。结果:积雪草苷的回归方程为:Y=34 958X+154 822 R2=0.999 1(n=8),羟基积雪草苷的回归方程为:Y=114 318X-21 002 R2=0.999 2(n=8),结果表明线性关系与分离度良好,平均加样回收率为99.98%、100.53%,RSD为1.86%、2.31%。结论:该方法稳定性、重复性良好,可做为天胡荽属药用资源的评价方法之一,实验结果为野生与栽培资源的开发与利用奠定了基础。

关 键 词:天胡荽属植物  积雪草苷  羟基积雪草苷  HPLC

Determination of Asiaticoside and Madecassoside in Hydrocotyle
Dong Guantao , Song Liangke , Tang Hao , Wang Yan , Wang Xiaoning.Determination of Asiaticoside and Madecassoside in Hydrocotyle[J].Chinese Wild Plant Resources,2012,31(2):47-50.
Authors:Dong Guantao  Song Liangke  Tang Hao  Wang Yan  Wang Xiaoning
Institution:(Life Science and Engineering College of Southwest Jiaotong University,Chengdu 610031,China)
Abstract:Objective: To establish a stable method for the content determination of asiaticoside and madecassoside in Hydrocotyle.And lay a foundation for the further study.Method: The HPLC was performed using Symmetry C18 column(4.6 mm×250 mm,5 μm) and acetonitrile-water(29∶71) as the mobile phase.The flow rate was 0.8mL/min,the detection wavelength was 210 nm,and the column temperature was 30 ℃.The external standard method was used as quantitative analysis.Results: the regression equation of asiaticoside was Y=34 958X+154 822 R2=0.999 1(n=8),the regression equation of madecassoside was Y=114 318X-21 002 R2=0.999 2(n=8);the average recovery was 99.98%、100.53%,RSD was 1.86%、2.31%.Conclusion: The method is stable and reliable.It can be used for quality control of Hydrocotyle.And the result provides scientific basis for exploiting wild and plant resources.
Keywords:Hydrocotyle  asiaticoside  madecassoside  HPLC
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号