豆腐柴叶提取低酯果胶的研究

豆腐柴叶含有丰富的果胶。文中结合盐酸浸提法的基础上,采用醇氨降酯法提取豆腐柴叶的果胶并检测,提取率为部颁19.5%,pH值为2.9,水分和灰分分别为5.13%和8.67%,半乳糖含量为65%,酯化度为38.5%,所提取的低酯果胶的基本性质与国标检测标准接近。果胶成品经AB-8树脂柱纯化后,所提果胶的成分经硅胶板薄层层析初步测定,果胶多糖成分为葡萄糖,果糖,D-甘露糖3种,果胶的溶解度与温度呈正相关性,pH值对其溶解度影响不大。

Low-ester Pectin Extraction from Premna Leaves

Premna leaves are rich in pectin.Based on hydrochloric acid extraction method,alcohol and ammonia ester lowering method was adopted to extract Premna leaves＇ pectin.The extraction rate was 19.5%,and the testing results showed that its pH value was 2.9;moisture,ash and galactose content 5.13%,8.67% and 65% respectively.After purified by AB-8 resin column,silica gel thin-layer chromatography showed that the pectin polysaccharides were composed of glucose,fructose and D-mannose.There existed a positive correlation between the pectin＇s solubility and temperature,pH value appearing little affect on its solubility,though.