UPLC-UV法测定不同产地青蒿中青蒿素的含量

目的:建立快速简便检测青蒿素的超高效液相-紫外(UPLC-UV)法,并对不同产地青蒿中青蒿素的含量进行检测。方法:色谱柱Agilent Eclipse Plus C18(2.1 mm×50 mm,1.8μm),流动相乙腈/水(45/55),流速1.0mL/min,柱温28℃,波长200 nm。结果:该方法对青蒿素的分离度较好,保留时间缩短为1.5 min。并且,整个分析过程可以在2 min内完成。线性范围0.101 17～10.117μg,进样量与峰面积线性相关,A=109.4C+6.7026,R2=0.9 993(n=9),加样回收率为99.3%(RSD=2.6%,n=6)。结论:UPLC-UV法分析时间短、样品前处理简单、精密度、稳定性、加样回收率等符合分析检测要求,对于青蒿中青蒿素的含量能进行快速准确的分析。

Determination of Artemisinin in Herba Artemisia Annuae from Different Locations by UPLC-UV

Objective:To establish a simple,rapid and accurate method for the determination of artemisinin in Artemisia annua from different locations by Ultra Performance Liquid Chromatography-Ultraviolet(UPLC-UV).The separation was performed on Agilent Eclipse Plus C18 column(2.1 mm× 50 mm,1.8 μm).Methods:The mobile phase consisted of acetonitrile / water(45 / 55) at the flow rate of 1.0 mL/ min.The detection wavelength was set at 200 nm and column temperature was 28 ℃.Results:Comparing with the other methods,separation of artemisinin is better and retention time(1.5 minutes) is shorter.Furthermore,the determination by UPLC would completed in 2 minutes.The linear rang of artemisinin was 0.10 117~10.117 μg,A = 109.4 C + 6.7 026,R2 = 0.9 993(n=9) by UPLC Method.The average recovery was 99.3%(RSD = 2.6 %,n=6).Conclusion: UPLC-UV is a simple,rapid,accurate and stable method for the determination of artemisinin in Artemisia annua.