| 野生资源植物金荞麦离体快繁技术研究 |
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| 引用本文： | 黄仁术,王娟,卫欢欢,马振鹏.野生资源植物金荞麦离体快繁技术研究[J].中国野生植物资源,2010,29(5):60-63. |
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| 作者姓名： | 黄仁术 王娟 卫欢欢 马振鹏 |
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| 作者单位： | 1. 皖西学院植物细胞工程安徽省工程技术研究中心
2. 皖西学院化学与生命科学系,安徽,六安,237012 |
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| 基金项目： | 六安市定向委托皖西学院市级研究项目《皖西野生资源植物金荞麦快繁技术研究》 |
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| 摘 要： | 针对金荞麦野生资源开发利用情况,试验以茎段为外植体,系统地探讨了以组织培养为手段进行快速繁殖的途径。结果发现：①外植体用HgCl2的消毒时间为6 min;②初代培养时培养基可选择MS＋1.0 mg/L6-BA＋0.5 mg/L NAA＋30 g/L蔗糖;③继代培养时培养基可选择MS＋1.0 mg/L 6-BA＋0.2 mg/L NAA＋30 g/L蔗糖;④生根培养时培养基可选择MS＋0.5 mg/L NAA＋20 g/L蔗糖。
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| 关 键 词： | 金荞麦 组织培养 茎段 |
Study on Rapid Propagation Technique in Vitro of Wild Resources Plant Fagopyrum dibotrys |
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| Huang Renshu,Wang Juan,Wei Huanhuan,Ma Zhenpeng.Study on Rapid Propagation Technique in Vitro of Wild Resources Plant Fagopyrum dibotrys[J].Chinese Wild Plant Resources,2010,29(5):60-63. |
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| Authors: | Huang Renshu Wang Juan Wei Huanhuan Ma Zhenpeng |
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| Institution: | 1.Engineering Technology Research Center of Plant Cell Engineering,Anhui Province;2.Chemistry and Life Science Department of West Anhui University,Lu＇an 237012,China) |
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| Abstract: | According to the exploitative situation of Fagopyrum dibotrys＇ wild resources,the methods of rapid propagation by the way of tissue culture were discussed systematically.The stem segment were chosed as the explants in the rapid propagation.The results showed that： ①the optimum sterilization time of the explants was 6 minutes by HgCl2;②MS＋1.0 mg/L 6-BA＋0.5 mg/L NAA＋30 g/L sucrose was the optimum medium for induction;③MS＋1.0 mg/L 6-BA＋0.2 mg/L NAA＋30 g/L sucrose was the optimum medium for reduplication;④MS＋0.5 mg/L NAA＋20 g/L sucrose was optimum rooting medium. |
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| Keywords: | Fagopyrum dibotrys tissue culture stem segment |
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