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| 微孔草籽油的超临界CO2流体萃取及HPLC—MS分析 | |
| 引用本文： | 曹越,赵先恩,索有瑞.微孔草籽油的超临界CO2流体萃取及HPLC—MS分析[J].中国野生植物资源,2009,28(6):52-56. |
| 作者姓名： | 曹越 赵先恩 索有瑞 |
| 作者单位： | 1. 中国科学院西北高原生物研究所,青海,西宁,810008;中国科学院研究生院,北京,100049;青海大学医学院药学系,青海,西宁,810001 2. 中国科学院西北高原生物研究所,青海,西宁,810008;山东省分析测试中心,山东,济南,250014 3. 中国科学院西北高原生物研究所,青海,西宁,810008 |
| 基金项目： | 中国科学院"西部之光"人才培养计划项目 |
| 摘 要： | 利用超临界CO2萃取微孔草籽油，并对籽油进行了HPLC／MS分析。实验确定的最佳超临界CO2流体萃取条件是：萃取温度45℃，萃取压力20MPa，CO2流量为35-40kg／h，萃取时间120min，在此条件下白刺籽油的萃取率为16．12％。利用HPLC／MS对微孔草籽油分析，发现其不饱和脂肪酸的相对含量高达73．19％。比较了超临界CO2萃取微孔草籽油油样和石油醚萃取微孔草籽油油样的理化性质，发现超临界CO2流体萃取的籽油质量优于传统溶剂萃取的籽油。 |
| 关 键 词： | 微孔草 超临界CO2萃取 不饱和脂肪酸 HPLC/MS分析 |
Supercritical CO_2 Fluid Extraction of Microula sikkimensis Hemsl. Seed Oil and HPLC/MS Analysis |
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| Institution: | Cao Yue, Zhao Xianen, Suo Yourui ( 1. Northwest Institute of Plateau Biology, China Academy of Sciences, Xining 810008, China; 2. Graduate University of China Academy of Sciences, Beijing 100049, China; 3. Department of Pharmacy, Medicine College of Qinghai University ,Xining 810001, China; 4. Shandong Analysis and Test Center, Jinan 250014, China) |
| Abstract: | Microula sikkimensis Hemsl. seed oil was obtained by supercritical carbon dioxide extraction and its fatty acid composition was analyzed by HPLC/MS. The results showed that the optimum parameters were as follows ： pressure 20 MPa, temperature 45 ℃, CO2 flow rate 35 - 40 kg/h and dynamic extraction time 120 min. The oil yield was 16.12% in the optimum conditions. The oil contained abundant unsaturated fatty acids. The content of unsaturated fatty acid was 73.19%. The oil extracted with supercritical CO2 was superior to that with petroleum ether. |
| Keywords: | Microula sikkimensis Hemsl supercritical CO_2 extraction unsaturated fatty acid HPLC/ MS analysis |
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