| 传统中药何首乌组织培养的初步研究 |
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| 引用本文： | 袁红霞,王金虎,陈德燕.传统中药何首乌组织培养的初步研究[J].中国野生植物资源,2006,25(3):56-58. |
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| 作者姓名： | 袁红霞 王金虎 陈德燕 |
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| 作者单位： | 苏州科技学院生物系,江苏,苏州,215009 |
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| 摘 要： | 主要对何首乌块根和幼茎进行了愈伤组织培养,并对何首乌不同外植体的诱导时间进行了比较.结果表明：诱导何首乌块根产生愈伤组织的最佳激素配比是0.1 mg/L 6-BA＋3.0 mg/LIAA＋0.5 mg/L 2,4-D,且这3种激素的作用主次为2,4-D＞IAA＞6-BA;在此次实验中还比较了不同消毒剂及其组合对何首乌块根的消毒效果,结果表明：70%的酒精＋0.1%HgCl2具有很好的消毒作用.
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| 关 键 词： | 组织培养 何首乌 愈伤组织 |
| 文章编号： | 1006-9690(2006)03-0056-03 |
| 修稿时间： | 2005年11月18 |
Preliminary Research on Tissue Culture of Polygonum multiforum |
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| Yuan Hongxia,Wang Jinhu,Chen Deyan.Preliminary Research on Tissue Culture of Polygonum multiforum[J].Chinese Wild Plant Resources,2006,25(3):56-58. |
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| Authors: | Yuan Hongxia Wang Jinhu Chen Deyan |
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| Abstract: | The callus culture of Polygonum multiforum Thunb was induced.The results showed that the growth was better in MS medium which contained 0.1 mg/L 6-BA 3.0 mg/L IAA 0.5 mg/L 2,4-D and the order of their efficiencies was 2,4-D>IAA>6-BA.the disinfection effects of different regents were also studied,and the results indicated that 70%alcohol 0.1% corrosive sublimate is the best combination. |
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| Keywords: | tissue culture Polygonum multiflorum callusd |
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