| 补骨脂素—UVA对端粒DNA的直接作用及其原子力显微镜研究 |
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| 引用本文： | 曹恩华,陈艾,张晓嫣,孙雪光,秦静芬,刘大格,白春礼.补骨脂素—UVA对端粒DNA的直接作用及其原子力显微镜研究[J].激光生物学报,2001,10(1):1-6. |
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| 作者姓名： | 曹恩华 陈艾 张晓嫣 孙雪光 秦静芬 刘大格 白春礼 |
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| 作者单位： | 1. 中国科学院生物物理所,
2. 中国科学院化学所, |
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| 基金项目： | 中国科学院“九五”重大基础支持项目! (KJ 95 1 AL 5 0 2 0 2 ) |
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| 摘 要： | HeLa细胞DNA，经Rsal(1250U)和HinF1(1250U)内切酶酶解、Bio-gel P-2柱分离，得到端粒DNA。原子力显微镜（AFM）的直接观测结果表明在UVA照射下，补骨脂素可结合到端粒DNA的特殊位置，其光交链产物随照射时间增长而增加。与此同时，P53表达和端粒酶活性发生变化。纯化的端凿DNA片段在AFM下呈带尾的T－环结构（T-loop）。按其高度判断T－环部分为双链DNA结构，T－环与尾部交接处具有三链或四链结构特征。
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| 关 键 词： | 补骨脂素 HeLa细胞 端粒DNA T－loop 原子力显微镜 |
| 文章编号： | 1007-7146(2001)01-0001-06 |
| 修稿时间： | 2000年10月18 |
Direct Effects of Psoralen-photosensitization on Telomeres and its Studies of Atomic Force Microscopy |
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| CAO En-hua,CHEN Ai,ZHANG Xiao-yan,SUN Xue-guang,QIN Jing-fen,LIU Da-ge,BAI Chun-Li.Direct Effects of Psoralen-photosensitization on Telomeres and its Studies of Atomic Force Microscopy[J].ACTA Laser Biology Sinica,2001,10(1):1-6. |
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| Authors: | CAO En-hua CHEN Ai ZHANG Xiao-yan SUN Xue-guang QIN Jing-fen LIU Da-ge BAI Chun-Li |
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| Institution: | CAO En-hua1,CHEN Ai1,ZHANG Xiao-yan1,SUN Xue-guang1,QIN Jing-fen1,LIU Da-ge2,BAI Chun-Li2 |
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| Abstract: | DNA from HeLa cells was cleaved with Rasl and Hinf ,and then purified by Bio-Gel P-2 column.Results showed that upon near-UV-irradiation,psoralen could be targeted to the repetitive sequences of telomeres and the psoralen cross-linking products increased with increase of irradiation time.At same time,the changes of the expression of P53 oncogene and telomerase was were observed. Large duplex loops (T-loop) with a tail were observed directly by AFM.The loop-tail junction position was assumed to be a tri-or tetra- strand DNA structure according to its apparent height. |
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| Keywords: | psoralen HeLa cells telomeric DNA T loop atomic force microscopyCAO En-hua:Male was born in Taizhow of Jiangsu Province China in Nov 1940 Graduated from Shanghai Medical University in 1965 and since then he has been working in Institute of Bi |
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