| 离子束介导GUS基因转化拟南芥种子的研究 |
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| 引用本文： | 谷运红,秦广雍,余增亮.离子束介导GUS基因转化拟南芥种子的研究[J].激光生物学报,2006,15(4):337-341. |
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| 作者姓名： | 谷运红 秦广雍 余增亮 |
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| 作者单位： | 1. 郑州大学物理工程学院,河南,郑州,450052
2. 中科院等离子体物理研究所,安徽,合肥,230031 |
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| 基金项目： | 国家自然科学基金项目(10505018) |
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| 摘 要： | 以拟南芥种子为研究对象,研究离子束介导GUS基因导入拟南芥种子的转化效果。结果表明:直接注入干种子后进行转化,未得到瞬间表达的结果。而注入玻璃化保护后的2天龄幼苗,再进行转化,转化效果明显提高,得到了大约10%的瞬间表达。揭示对于像拟南芥这样的小粒种子,采用幼苗注入后转化,效果可能会更好一些。
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| 关 键 词： | 离子束 转化 拟南芥 |
| 文章编号： | 1007-7146（2006）04-0337-05 |
| 收稿时间： | 2006-03-14 |
| 修稿时间： | 2006年3月14日 |
A Study on Transformation Effect of GUS Gene Introduced into Arabidopsis Seeds Mediated by Ion Beam |
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| GU Yun-hong,QIN Guang-yong,YU Zeng-liang.A Study on Transformation Effect of GUS Gene Introduced into Arabidopsis Seeds Mediated by Ion Beam[J].ACTA Laser Biology Sinica,2006,15(4):337-341. |
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| Authors: | GU Yun-hong QIN Guang-yong YU Zeng-liang |
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| Abstract: | Transformation effection of GUS gene introduced into Arabidopsis seeds mediated by ion beam was studied.The results were that transient expression had not obtained after transformating dry seeds implanted.But if 2-day-seedling was implanted,and then dipped into DNA solution,the transformation effect was increased evidently.10 % transient expression was obtainted.The results tell us small seeds such as arabidopsis seeds maybe give better transformation effect when 2-day-seedling were implanted firstly and then dipped into DNA solution. |
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| Keywords: | ion beam transformation Arabidopsis |
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