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新型湘云金鳙遗传多样性的RAPD及微卫星分析
引用本文:严斌,彭亮跃,谢伟民,肖亚梅,刘少军,周工健.新型湘云金鳙遗传多样性的RAPD及微卫星分析[J].激光生物学报,2011,20(1):54-60,73.
作者姓名:严斌  彭亮跃  谢伟民  肖亚梅  刘少军  周工健
作者单位:1. 湖南师范大学生命科学学院教育部蛋白质化学和鱼类发育生物学重点实验室,湖南,长沙,410081
2. 湖南省宁远县第三中学,湖南,宁远,425600
基金项目:湖南省科技厅农业支撑计划项目
摘    要:湘云金鳙是本实验室经过近十年的工作选育出来的,该种鱼通体金红色,肉质鲜嫩,生长速度快,是一种很有价值的养殖鱼种和实验材料.随机选取12尾湘云金鳙(RBC)和12尾来自湘江流域的普通鳙鱼BC(作为对照)进行RAPD和微卫星分析.在优化RAPD检测条件基础上,从230个随机引物中筛选出来的45个扩增较好且多态性强的引物对这...

关 键 词:湘云金鳙  RAPD  微卫星  遗传多样性

RAPD and Microsatellite Analysis of Red Bighead Carp (Aristichthys nobilis)
YAN Bin,PENG Liang-yue,XIE Wei-min,XIAO Ya-mei,LIU Shao-jun,ZHOU Gong-jian.RAPD and Microsatellite Analysis of Red Bighead Carp (Aristichthys nobilis)[J].ACTA Laser Biology Sinica,2011,20(1):54-60,73.
Authors:YAN Bin  PENG Liang-yue  XIE Wei-min  XIAO Ya-mei  LIU Shao-jun  ZHOU Gong-jian
Institution:YAN Bin~1,PENG Liang-yue~1,XIE Wei-min~2,XIAO Ya-mei~1,LIU Shao-jun~1,ZHOU Gong-jian~(1*) (1.Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China,College of Life Sciences,Hunan Normal University,Changsha 410081,Hunan,China,2.The Third Middle School of Ningyuan County,Ningyuan 425600,China)
Abstract:Genetic variation was comparatively analyzed between the Red bighead carp(RBC) population and the bighead carp(BC) population of aquaculture strains originating from xiangjiang river used as the control,using random amplified polymorphic DNA(RAPD) assay and microsatellite analysis.The specific fragments for RBC and BC population was detected by S20 and S46 respectively:The average genetic distances of BC ranged from 0.04 to 0.13 with a mean of 0.075.And the average genetic distances of RBC ranged from 0.02 ...
Keywords:RAPD
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