LED照射对培养SW1353细胞MPP-3与MMP-13的影响

在骨关节疾病中,基质金属蛋白酶(matrix metalloproteinase,MMP)对关节软骨缺损的机理扮演着重要的角色。为了进一步明确低强度激光在关节软骨缺损中的治疗作用,应用细胞因子IL-1β与TNF-α刺激培养SW 1353细胞复制炎症细胞模型,观察发光二极管(LED)照射对培养细胞的生存率和死亡率的影响、细胞活性的影响、以及对MMP-3和MMP-13的调节作用。实验结果显示,10μg/L IL-1β与20μg/L TNF-α对培养细胞生存率无明显影响,而细胞活性明显下降(P<0.01),培养液中MMP-3和MMP-13含量明显上升(P<0.01,P<0.05);LED照射后可见与相应的模型组比较细胞死亡率下降(P<0.01)、培养液中MMP-3和MMP-13含量显著性降低在(P<0.01,P<0.05)。研究表明,LED照射对炎症因子刺激的SW 1 353细胞的MMP过多表达具有抑制性作用,可能对于关节软骨缺损性疾病的LED照射治疗起一定的指导意义。

Effects of LED Irradiation on the Expression of MMP-3 and MMP-13 in the SW1353 Cells in vitro

Matrix metalloproteinase(MMP) plays an active role in remodeling cartilage in osteoarthritic cartilage.To find an effective method of prevention of osteoclasia,this in vitro study focuses on the expression of MMP-3 and MMP-13 in the SW1353 cells by LED irradiation.The human chondrosarcoma cell line SW1353 were stimulated with the proinflammatory cytokine IL-1beta or tumor necrosis factor-alpha(TNF-alpha),and were received the irradiation of LED(632 nm,4 mW/cm2).The cell count was assessed over a 96-hour period by using Trypan blue dye exclusion assay,and the cell activity was evaluated with a Cell Counting Kit-8 Assays.The subsequent expression of MMP-3 and MMP-13 was quantified.The results show that the cultural cell activity was decreased,and the expression of MMP-3 and MMP-13 was increased by being stimulated with IL-1beta or TNF-alpha.After received LED irradiation,the death rate of cultural cell was increased and the expression of MMP-3 and MMP-13 was decreased significantly.The present study concluded that particular LED irradiation stimulates SW1353 cell proliferation activity and inhibit the MMP-3 and MMP-13 enzymatic activity.These findings might be clinically relevant,indicating that the low power laser irradiation treatment is likely to achieve the repair of articular cartilage in clinic.