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| 曲酸高产菌株的诱变选育 | |
| 引用本文： | 赵鑫,赵金,吴思国,胡翠敏,赵宗保.曲酸高产菌株的诱变选育[J].激光生物学报,2010,19(4):542-545. |
| 作者姓名： | 赵鑫 赵金 吴思国 胡翠敏 赵宗保 |
| 作者单位： | 1. 中国科学院大连化学物理研究所,辽宁,大连,116023;中国科学院研究生院,北京,100039 2. 中国科学院大连化学物理研究所,辽宁,大连,116023 3. 中国科学院大连化学物理研究所,辽宁,大连,116023中国科学院研究生院,北京,100039 |
| 基金项目： | 中国科学院知识创新工程领域前沿项目 |
| 摘 要： | 以紫外线和氮离子注入作为诱变手段对曲酸产生菌黄曲霉CICC2242进行诱变处理，筛选出一株高产菌株N83。曲酸产量由初始的12．4g／L提高到19．4g／L，提高了56．5％；遗传稳定性实验结果表明，N83遗传性状稳定良好。该结果表明氮离子注入诱变是获得高产曲酸的有效途径。 |
| 关 键 词： | 曲酸 紫外线 氮离子注入 诱变 |
Mutagenesis bf Strain with High Yield of Kojic Acid |
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| ZHAO Xin,ZHAO Jin,WU Si-guo,HU Cui-min,ZHAO Zong-bao.Mutagenesis bf Strain with High Yield of Kojic Acid[J].ACTA Laser Biology Sinica,2010,19(4):542-545. | |
| Authors: | ZHAO Xin ZHAO Jin WU Si-guo HU Cui-min ZHAO Zong-bao |
| Institution: | 1. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, China; 2. Graduate university of Chinese Academy of Sciences, Beijing 100039, China) |
| Abstract: | The kojic acid-producing strain Aspergillus flavus CICC 2 242 was mutagenized using UV radiation and N^＋ implantation, and its mutant strain A. flavlts N83 was obtained. The mutant strain produced kojic acid at 19.4 g/L, which was 56.5 % higher than that with A. flavus CICC 2 242 at 12.4 g/L. Moreover, A. flavus N83 showed good genetic stability. The results showed that N^＋ implantation was an effective method to generate better kojic acid producer. |
| Keywords: | kojic acid UV radiation N^＋ implantation mutagenesis |
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