| 枯草杆菌SOD高产菌株的诱变选育及产酶条件研究 |
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| 引用本文： | 陈晓琳,唐欣昀,周隆义.枯草杆菌SOD高产菌株的诱变选育及产酶条件研究[J].激光生物学报,2005,14(3):173-176. |
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| 作者姓名： | 陈晓琳 唐欣昀 周隆义 |
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| 作者单位： | 安徽农业大学生命科学学院,中国,安徽,合肥,230036 |
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| 基金项目： | 安徽农业大学校长青年基金 |
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| 摘 要： | 本实验采用低能氮离子注入技术对枯草芽孢杆菌(Bacillus subtilis)进行辐照诱变处理，选育出一株SOD高产菌株(编号为BsB8)。其生物量略高于出发菌株、SOD产量达3439．3U／g湿菌体，为实验出发菌的1．88倍。该菌株最佳产酶条件为：起始pH为8．2，装液量为150ml／500ml，接种量为1．5％；添加Mn^2 的培养基可显著提高SOD酶活。
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| 关 键 词： | 枯草杆菌 氮离子注入 SOD 产酶条件 |
| 文章编号： | 1007-7146(2005)03-173-04 |
| 收稿时间： | 2005/2/28 |
| 修稿时间： | 2005年2月28日 |
The Breeding of the SOD High-producing Strain from Bacillus stbtilis and Studies on Culture Conditions for SOD Production |
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| CHEN Xiao-lin,TANG Xin-yun,ZHOU Long-yi.The Breeding of the SOD High-producing Strain from Bacillus stbtilis and Studies on Culture Conditions for SOD Production[J].ACTA Laser Biology Sinica,2005,14(3):173-176. |
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| Authors: | CHEN Xiao-lin TANG Xin-yun ZHOU Long-yi |
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| Abstract: | After the strain of Bactillus subtilis was treated with low energy ion beam implantation, a high-producing mutant was obtained. The content of SOD reached 3429.3 μ/g fresh cells, which was 1.88 times of original strains. The tests of culture conditions showed that the optimal pH was 8.2, the optimal liquid content was 150 ml/500 ml, the optimal inoculation content was (1.5) %. The content of SOD from B. subtilis obviously increased in the Mn~(2+) culture medium. |
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| Keywords: | Bactillus subtilis ion beam implantation superoxide dismutase mutation culture conditions |
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