| 洋甘菊组织培养及植株再生 |
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| 引用本文： | 杨俊杰,袁艺,王小娟,何兵.洋甘菊组织培养及植株再生[J].激光生物学报,2009,18(6):825-829. |
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| 作者姓名： | 杨俊杰 袁艺 王小娟 何兵 |
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| 作者单位： | 1. 安徽农业大学生命科学学院,安徽,合肥,230036
2. 合肥一中,安徽,合肥,230061 |
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| 基金项目： | 合肥市科技局重点资助项目 |
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| 摘 要： | 洋甘菊种子灭菌后,置于MS 培养基上发芽形成无菌苗,发芽率可达80 %.适合洋甘菊种子的灭菌的方法是:75 %酒精30 s+3.5 %次氯酸钠15 min+2 %次氯酸钠10 min.在研究范围内,洋甘菊不定芽增殖的最佳培养基为MS+6-BA 1.5 mg/L+NAA 0.5 mg/L,碳源以蔗糖浓度3 %最佳.适宜洋甘菊生根的最佳培养基为MS+IBA 1.0 mg/L+NAA 0.5 mg/L+6-BA 0.2 mg/L.
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| 关 键 词： | 洋甘菊 组织培养 激素 碳源 |
Tissue Culture and Plant Regeneration of Chamomilla recatita L. |
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| YANG Jun-jie,YUAN Yi,WANG Xiao-juan,HE Bing.Tissue Culture and Plant Regeneration of Chamomilla recatita L.[J].ACTA Laser Biology Sinica,2009,18(6):825-829. |
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| Authors: | YANG Jun-jie YUAN Yi WANG Xiao-juan HE Bing |
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| Abstract: | Seeds of Chamomilla recutita L. disinfected were cultured as explants in MS medium. Hormones combination and carbon source of Chamomilla recutita L. were studied. The results showed that the best medium for inducting lateral shoots was MS+6-BA 1.5 mg/L+NAA 0.5 mg/L. The optional concentration was 3 % sucrose. The suitable rooting culture medium was MS+IBA 1.0 mg/L+NAA 0.5 mg/L+6-BA 0.2 mg/L. |
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| Keywords: | Chamomilla recutita L tissue culture hormones carbon source |
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