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抗虫杀菌融合基因表达载体的构建及微束激光转化植物研究
引用本文:张楠,陈正华,刘桂珍,陈凌娜,曲延英.抗虫杀菌融合基因表达载体的构建及微束激光转化植物研究[J].激光生物学报,2007,16(2):200-207.
作者姓名:张楠  陈正华  刘桂珍  陈凌娜  曲延英
作者单位:1. 新疆农业大学农学院,新疆,乌鲁木齐,830052
2. 甘肃亚盛集团博士后科研工作站北京分站,北京,100101
摘    要:将苏云金芽孢杆菌伴孢晶体蛋白的基因(Bacillus thuringiesis,简称Bt)通过甘氨酸接头(Gly4Ser)3与一种人工合成的抗菌肽(antimicrobial peptides,AMP)基因与相融合,编码一种新的杀虫,并具有抗菌的蛋白。把融合基因(NAMP-Bt)连接到原核表达载体pET-28a和植物表达载体pBI-121上,经过限制性酶切分析和PCR鉴定,结果表明含有融合基因的原核和真核重组表达质粒均已构建成功,并将该融合基因转入烟草,已获得抗性小植株。

关 键 词:苏云金芽孢杆菌伴孢晶体蛋白基因  抗菌肽基因  原核表达载体  植物表达载体  限制性酶切分析  PCR鉴定
文章编号:1007-7146(2007)02-0200-08
收稿时间:2006-07-30
修稿时间:2006年7月30日

Construction of Expression Vector with Anti-insect and Antifungal Gene and Study on Transformation Plant by Laser Microbeam
ZHANG Nan,CHEN Zheng-hua,LIU Gui-zhen,CHEN Ling-na,QU Yan-ying.Construction of Expression Vector with Anti-insect and Antifungal Gene and Study on Transformation Plant by Laser Microbeam[J].ACTA Laser Biology Sinica,2007,16(2):200-207.
Authors:ZHANG Nan  CHEN Zheng-hua  LIU Gui-zhen  CHEN Ling-na  QU Yan-ying
Institution:1. Agronomy College, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China; 2. Postdoctoral Scientific Research Station of Gansu Yasheng Industrial (Group
Abstract:Antifungal protein gene and Bacillus thuringiesis gene were ligated by the linker of(Gly4Ser)_3,which encode a new anti-insect and antifungal protein.The fusion gene(NAMP-Bt) were ligated into prokaryotic expression vector and plant expression vector.These recombinant plasmids pET-NAMP-Bt and pBI-NAMP-Bt were confirmed by restriction enzyme and PCR analysis and the results showed that these recombinant plasmids were constructed successfully.The fusion gene was induced into tobacco,we had obtained transgenic tobacco.
Keywords:Bacillus thuringiesis  antimicrobial peptides gene  prokaryotic expression vectors  plant expression vectors  analysis of restriction enzyme  PCR analysis
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