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植物总DNA样品的快速制备
引用本文:王艳,李韶山,刘颂豪.植物总DNA样品的快速制备[J].激光生物学报,2000,9(1):79-80,F003.
作者姓名:王艳  李韶山  刘颂豪
作者单位:暨南大学生命科学和技术学院,广州
基金项目:广东省自然科学基金,广东省高教厅及广州市科委基础研究项目
摘    要:利用Qiagen微量植物DNA提取试剂盒,在1小时内即可从植物组织获得总DNA,提取过程中勿需酚/氯仿和SDS抽提,操作简便、快捷。所得DNA样品的OD260/OD280值在1.7-1.9之间。样品纯度高;该样品不含PCR反应抑制剂及其他酶反应抑制剂,可被各种限制性内酶完全降解,适合于PCR、印迹、RAPD、AFLP和RFLP分析等各种下游应用。

关 键 词:DNA提取  水稻  花生  玉米  植物

Quick Preparation of Total DNA from Plants
WANG Yan,LI Shaoshan,LIU Songhao.Quick Preparation of Total DNA from Plants[J].ACTA Laser Biology Sinica,2000,9(1):79-80,F003.
Authors:WANG Yan  LI Shaoshan  LIU Songhao
Institution:WANG Yan 1 LI Shaoshan 2 LIU Songhao 2
Abstract:A fast and simple way to isolate total DNA from up to 100mg of plant tissue was developed in the present paper. By using the DNeasy Plant Mini Kit, high quality of total DNA from plant was yielded in less than 1 hour. Purification required no phenol or chloroform extraction and involved minimal handling. This made the kit ideal for simultaneous processing of multiple samples. Plant DNA isolated with this method could be completely digested by restriction endonucleases, and was ready for use in all kinds of downstream applications including PCR, blotting, analysis of RAPD, AFLP and RFLP.
Keywords:DNA isolation  rice (Oryza sativa)  peanut (Arachis hypogaea)  maize (Zay mays)
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