| 基于双光子激发的荧光相关谱测量 |
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| 引用本文： | 刘亚丰,陈同生,骆清铭,曾绍群.基于双光子激发的荧光相关谱测量[J].激光生物学报,2003,12(6):421-425,430. |
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| 作者姓名： | 刘亚丰 陈同生 骆清铭 曾绍群 |
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| 作者单位： | 华中科技大学生物医学光子学教育部重点实验室,中国湖北,武汉,430074 |
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| 基金项目： | 国家自然科学基金重点项目(59836240)，国家自然科学基金项目(30170306)，973前期研究项目(2001CCA04100)。 |
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| 摘 要： | 本文利用多光子激发激光扫描显微镜的部分光路和探测器．建立了双光子荧光相关谱系统(Two-Photo Fluorescence Correlation Spectroscopy．简称TP-FCS)。利用TP-FCS系统观察到了“光子爆发”现象．实现了染料分子的双光子激发，测量出若丹明B染料分子在蔗糖溶液中的扩散系数。实验证明该系统具有操作简便、可靠性高，费用低廉等等点，可实现单分子检测。
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| 关 键 词： | 双光子激发 荧光相关谱 光子爆发 单分子检测 |
| 文章编号： | 1007-7146(2003)06-0421-05 |
| 收稿时间： | 2003/4/28 |
Fluorescence Correlation Spectroscopy Based upon Two-Photon Excitation |
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| Abstract: | A Two-Photon Fluorescence Correlation Spectroscopy (TP-FCS) system was set up dependent on a part of optical block and detector of the multi-photon laser scanning microscope. The phenomenon "photon-burst" was observed and single rhodamine B molecule was excitated from the TP-FCS system. Meanwhile, the diffusion coefficient of rhodamine B molecule in sucrose aqueous solution was calculated. It was proved that this system had the advantages of handy operation, low cost and high reliability and could be used to detect single molecule. |
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| Keywords: | two-photon excitation fluorescence correlation spectroscopy photon-burst single molecule detection |
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