| 利用微卫星标记分析东平湖黄颡鱼的遗传多样性 |
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| 引用本文： | 马洪雨,姜运良,郭金峰,岳永生.利用微卫星标记分析东平湖黄颡鱼的遗传多样性[J].激光生物学报,2006,15(2):136-139. |
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| 作者姓名： | 马洪雨 姜运良 郭金峰 岳永生 |
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| 作者单位： | 山东农业大学动物分子遗传育种实验室,山东,泰安,271018 |
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| 基金项目： | 国家科技部资助项目(04033810)，山东省科技厅资助项目(04038335) |
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| 摘 要： | 采用27对鲤微卫星引物对山东东平湖黄颡鱼进行全基因组扫描,结果有19对引物能获得稳定的扩增条带,其中有6个微卫星位点具有多态性。对这6个位点的扩增产物进行分析,结果显示:6个位点共检测到22个等位基因,每个位点的等位基因数从2个到6个不等;平均基因纯合率为41.67%,平均多态信息含量(PIC)为0.488,平均杂合度为0.5833。这表明东平湖黄颡鱼种群结构合理,群体遗传多样性较丰富,种质资源处于安全状态。
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| 关 键 词： | 微卫星标记 黄颡鱼 遗传多样性 |
| 文章编号： | 1007-7146（2006）02-0136-04 |
| 收稿时间： | 2005-04-03 |
| 修稿时间： | 2005-12-05 |
Genetic Diversity Analysis on Pseudobagrus fulvidraco in Dongping Lake Using Microsatellite Markers |
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| MA Hong-yu,JIANG Yun-liang,GUO Jin-feng,YUE Yong-sheng.Genetic Diversity Analysis on Pseudobagrus fulvidraco in Dongping Lake Using Microsatellite Markers[J].ACTA Laser Biology Sinica,2006,15(2):136-139. |
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| Authors: | MA Hong-yu JIANG Yun-liang GUO Jin-feng YUE Yong-sheng |
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| Abstract: | Twenty-seven pairs of microsatellite loci were applied to scan genomic DNA of Pseudobagrus fulvidraco in Dong Ping Lake,Shandong Province.Electrophoresis analysis showed that 19 pairs of primers reproducibly produced clear bands,and,of them 6 were polymorphic.There were totally 22 alleles of these microsatellite loci,and the number of alleles per locus ranged from 2 to 6.The average homozygosity,average polymorphism information content and average heterozygosity was 41.67 %,0.488 and 0.5833,respectively.The results indicated that the population structure of Pseudobagrus fulvidraco in Dong Ping Lake was reasonable and the breed resource was in a safe status. |
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| Keywords: | microsatellite marker Pseudobagrus fulvidraco genetic diversity |
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