| 弱激光对脂质体介导的血管平滑肌细胞基因转染的影响 |
| |
| 引用本文： | 顾瑛,刘凡光,江亿平,朱建国,李峻亨.弱激光对脂质体介导的血管平滑肌细胞基因转染的影响[J].激光生物学报,1999,8(2):81-87. |
| |
| 作者姓名： | 顾瑛 刘凡光 江亿平 朱建国 李峻亨 |
| |
| 作者单位： | 解放军总医院激光科,北京,100853 |
| |
| 基金项目： | 国家自然基金委员会信息部资助项目 |
| |
| 摘 要： | 本研究采用阳离子脂质体介导外源基因转染体外培养的兔血管平滑肌细胞（ＳＭＣ），在基因转染过程中给予激光照射，用细胞化学染色方法测定基因转染阳性率。结果显示：用５１０．６ｎｍ激光于基因转染前，以功率密度１ｍｗ／ｃｍ２，能量密度２、４、６Ｊ／ｃｍ２和５ｍＷ／ｃｍ２，４、６Ｊ／ｃｍ２；及１０ｍＷ／ｃｍ２，２Ｊ／ｃｍ２进行照射均能显著提高基因转染率（ｐ＜０．０５）；于基因转染后即刻以功率密度１ｍＷ／ｃｍ２、能量密度２Ｊ／ｃｍ２和５ｍＷ／ｃｍ２、６Ｊ／ｃｍ２照射也能提高基因转染率（ｐ＜０．０５）。而用６２７．８ｎｍ激光照射对基因转染率无显著影响
|
| 关 键 词： | 弱激光 脂质体 基因转染 血管平滑肌细胞 |
Effect of Laser Irradiation on Liposome-mediated Gene Transfer into Vascular Smooth Muscle Cells |
| |
| GU Ying,LIU Fanguang,JIANG Yiping,ZHU Jianguo,LI Junheng.Effect of Laser Irradiation on Liposome-mediated Gene Transfer into Vascular Smooth Muscle Cells[J].ACTA Laser Biology Sinica,1999,8(2):81-87. |
| |
| Authors: | GU Ying LIU Fanguang JIANG Yiping ZHU Jianguo LI Junheng |
| |
| Abstract: | Complexing recombinant DNA with liposome is a convenient means of introducing foreign gene into cell and vascular.The efficiency of successful gene transfer,howere,is variable and low.The purpose of this study was to investigate the effect of low power laser irradiation on increasing liposome-mediated gene transfer into vascular smooth muscle cells (SMC).Culture SMC were irradiated by wavelength of 510.6nm and 627.8nm laser.The results indicated that 510.6nm laser irradiation with the power densities 1,5,10 mW/cm 2 and the energy densities of 2,4,6J/cm 2 could augment liposome-mediated gene transfer(p<0 05),but 627.8nm laser could not affect gene transfer. |
| |
| Keywords: | Low level Laser Vascular Smooth Muscle Cells Liposome Gene Transfer |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
