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文心兰原球茎液体增殖培养研究
引用本文:崔广荣,张子学,张从宇,孙涛.文心兰原球茎液体增殖培养研究[J].激光生物学报,2007,16(3):338-343.
作者姓名:崔广荣  张子学  张从宇  孙涛
作者单位:安徽科技学院植物科学系,安徽,凤阳,233100
基金项目:安徽省自然科学基金 , 安徽省教育厅自然科学基金
摘    要:以茎尖诱导形成的原球茎(protocorm-like bodies,PLBs)为外殖体,采用液体培养方式比较了不同浓度的激素配比、蔗糖浓度和添加不同量的新鲜椰汁对文心兰PLBs增殖的影响,并比较了相同培养基成分时液体培养PLBs增殖、分化成苗和固体培养PLBs增殖和分化成苗的差异。试验结果表明:不同浓度的外源激素及其配比对文心兰PLBs增殖生长影响较大,6-BA0.5 mg/L Ad 0.05 mg/L NAA0.05 mg/L的激素组合比较适合文心兰PLBs增殖;蔗糖浓度对文心兰PLBs增殖的影响也较大,适合文心兰PLB在液体培养条件下增殖的蔗糖浓度为7.5 g/L;添加5%新鲜椰汁不仅对文心兰PLBs增殖有促进作用,而且能改善PLBs的质量;适合文心兰PLBs增殖的培养基为MS 6-BA0.5 mg/L Ad 0.05 mg/L NAA0.05 mg/L 5%椰汁 蔗糖7.5 g/L。文心兰PLBs在5周内的增殖生长曲线呈倒"V"字形,第3周的增殖速度达最高峰,而固体培养基PLBs增殖速度较慢,生长曲线几乎成直线。液体增殖的PLBs分化成苗较固体培养增殖的PLBs差。

关 键 词:文心兰  原球茎(PLBs)  增殖  液体培养
文章编号:1007-7146(2007)03-0338-06
收稿时间:2006-04-08
修稿时间:2006-04-08

The Study on Oncidium PLBs Propagation in Liquid Culture
CUI Guang-rong,ZHANG Zi-xue,ZHANG Cong-yu,SUN Tao.The Study on Oncidium PLBs Propagation in Liquid Culture[J].ACTA Laser Biology Sinica,2007,16(3):338-343.
Authors:CUI Guang-rong  ZHANG Zi-xue  ZHANG Cong-yu  SUN Tao
Institution:Plant Science Department of Anhui Science and Technology University, Fengyang 233100, Anhui, China
Abstract:The oncidium PLBs(protocorm-like bodies) induced from stem tips were used as explants to be cultured in the liquid media which contain hormones,sucrose and coconut palm juice.The effects of different concentrations of hormones,sucrose and coconut palm juice on PLBs propagation were studied in this experiment.Under the same condition,the differences of PLB propagation and shoots regeneration in the liquid culture and solid culture were studied too.The results showed that the concentrations and combinations of hormones have great effect on oncidium PLBs propagation in the liquid culture.The best combination of hormones was 6-BA 0.5 mg/L Ad 0.05 mg/L.The concentrations of sucrose also have great effect on PLBs propagation.The suitable medium for oncidium PLBs propagation in the liquid culture was 7.5 g/L.Adding 5 ml/L coconut palm juice to the medium not only can elevate PLBs propagation coefficient but also improve the quality of oncidium PLBs.The best medium for oncidium PLBs propagation was MS 6-BA 0.5 mg/L Ad 0.05 mg/L sucrose 7.5 g/L coconut palm juice 5 mL/L.The PLBs cultured in this medium grew fast,less browning.The PLBs growth curve showed "V" in this liquid medium,while seemed be a line in the solid medium.However,the growth state of shoots regenerated from PLBs cultured in the liquid medium was worse than shoots regenerated from solid medium.
Keywords:Oncidium  protocorm-like body  propagation  liquid culture
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